Construction of novel vectors for transformation of Lentinula edodes using a chitin synthase gene promoter(GENETICS, MOLECULAR BIOLOGY, AND GENE ENGINEERING)
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概要
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Novel vectors (pLCHS-hph and pChG-bar) containing expression unit driving hph as a selectable marker gene by chitin synthase gene promoter were constructed for Lentinula edodes transformation. Expression of the hph gene in random selected transformants was confirmed by RT-PCR method. Thus, both vectors are useful for L. edodes transformation.
著者
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Sato Takahide
Department Of Bioproduction Science Faculty Of Horticulture Chiba University
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Hirano T
Kyoto Univ. Kyoto Jpn
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Sato Takako
The Deepstar Group Japan Marine Science And Technology Center
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Sato T
Institute Of Applied Biochemistry University Of Tsukuba
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SATO Toshitsugu
Iwate Biotechnology Research Center
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OKAWA Kumiko
Iwate Biotechnology Research Center
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HIRANO Tatsuya
Iwate Biotechnology Research Center
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Sato T
Iwate Biotechnology Research Center:department Of Biotechnology And Environmental Chemistry National
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Taniyama Hiroko
Department Of System Engineering Of Materials And Life Science Faculty Of Engineering Toyama Univers
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Horikawa Takashi
Collaborative Research Center For Advanced Science And Technology Osaka University
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Sato Tsutomu
Graduate School Of Science And Technology Faculty Of Agriculture Niigata University
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