Isolation of Industrial Strains of Aspergillus oryzae Lacking Ferrichrysin by Disruption of the dffA Gene(MICROBIAL PHYSIOLOGY AND BIOTECHNOLOGY)

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概要

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• Based on studies using laboratory strains, the efficiency of gene disruption in Aspergillus oryzae, commonly known as koji mold, is low; thus, gene disruption has rarely been applied to the breeding of koji mold. To evaluate the efficiency of gene disruption in industrial strains of A. oryzae, we produced ferrichrysin biosynthesis gene (dffA) disruptants using three different industrial strains as hosts. PCR analysis of 438 pyrithiamine-resistant transformants showed dffA gene disruption efficiency of 42.9%-64.1%, which is much higher than previously reported. Analysis of the physiological characteristics of the disruptants indicated that dffA gene disruption results in hypersensitivity to hydrogen peroxide. To investigate the industrial characteristics of dffA gene disruptants, two strains were used to make rice koji and their properties were compared to those of the host strains. No differences were found between the dffA gene disruptants and the host strains, except that the disruptants did not produce ferrichrysin. Thus, this gene disruption technique is much more effective than conventional mutagenesis for A. oryzae breeding.

• 社団法人日本生物工学会の論文
• 2008-11-25

著者

• Sato Takahide

  Department Of Bioproduction Science Faculty Of Horticulture Chiba University

• Sato Takako

  The Deepstar Group Japan Marine Science And Technology Center

• Sato T

  Institute Of Applied Biochemistry University Of Tsukuba

• Hatakeyama Makoto

  Local Independent Administrative Agency Iwate Industrial Research Institute

• Sakurai Hiroshi

  Local Independent Administrative Agency Iwate Industrial Research Institute

• SATO Toshitsugu

  Iwate Biotechnology Research Center

• Watanabe Hisayuki

  Iwate Biotechnology Research Center

• Uchimiya Hirofumi

  Iwate Biotechnology Research Center

• Sato T

  Iwate Biotechnology Research Center:department Of Biotechnology And Environmental Chemistry National

• Uchimiya Hirofumi

  Iwate Biotechnology Res. Center

• Sato Tsutomu

  Graduate School Of Science And Technology Faculty Of Agriculture Niigata University

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