The Tyrosinase-Encoding Gene of Lentinula edodes, Letyr, Is Abundantly Expressed in the Gills of the Fruit-Body during Post-Harvest Preservation
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概要
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The gill browning of Lentinula edodes fruit-bodies during preservation is thought to be due to melanin biosynthesis catalyzed by tyrosinase. We isolated a genomic DNA sequence and cDNA encoding a putative tyrosinase from the white rot basidiomycete Lentinula edodes (shiitake mushroom). The gene, named Letyr, consists of a 1,854-bp open reading frame interrupted by eight introns, and encodes a putative protein of 618 amino acid residues with an estimated molecular mass of 68 kDa. Amino acid residues known to be involved in copper-binding domains were conserved in the deduced amino acid residues of LeTyr. Transcriptional and translational expression of Letyr in the gills of the fruit-body increased during preservation after harvest. This correlation between Letyr expression and fruit-body preservation suggests that tyrosinase gene expression contributes to gill browning.
- 社団法人 日本農芸化学会の論文
著者
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Sato Takako
The Deepstar Group Japan Marine Science And Technology Center
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Yaegashi Kaori
Iwate Biotechnology Res. Center
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Sato T
Institute Of Applied Biochemistry University Of Tsukuba
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SAKAMOTO Yuichi
Iwate Biotechnology Research Center
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SATO Toshitsugu
Iwate Biotechnology Research Center
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Watanabe Hisayuki
Iwate Biotechnology Research Center
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Uchimiya Hirofumi
Iwate Biotechnology Research Center
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OKAWA Kumiko
Iwate Biotechnology Research Center
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HIRANO Tatsuya
Iwate Biotechnology Research Center
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KANDA Katsuhiro
Iwate Biotechnology Research Center
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TAKAHASHI Machiko
Iwate Biotechnology Research Center
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