Changes in Proteasome Levels in Spinach (Spinacia oleracea) Seeds during Imbibition and Germination
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概要
- 論文の詳細を見る
Proteasomes are the major cytosolic protease complexes responsible for energy-dependent and extra-lysosomal proteolysis. Ubiquitinated proteins are degraded by the 26S proteasome which is composed of a 20S proteasome and two regulatory complexes. Changes in the 20S and 26S proteasome activity levels and protein abundance in spinach seeds during imbibition and germination were examined by using glycerol density gradient centrifugation. The 26S proteasome activity level decreased transiently during imbibition, reached a minimum one day after starting imbibition, and then increased again. During the period of minimal accumulation, the protein being detected with an anti-26S proteasome antibody shifted to a lower sedimentation coefficient fraction. In contrast, the 20S proteasome activity level increased during imbibition, and remained high for two days. The change in the 20S proteasome level corresponded to the change in the 20S proteasome activity level.
- 社団法人日本農芸化学会の論文
- 1997-06-23
著者
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Tanaka Keiji
Division of Intensive and Coronary Care Unit, Nippon Medical School
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Sato Takahide
Department of Agricultural Chemistry, Faculty of Horticulture, Chiba University
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Nakagawa Hiroki
Department of Agricultural Chemistry, Faculty of Horticulture, Chiba University
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Sato Takahide
Department Of Bioproduction Science Faculty Of Horticulture Chiba University
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Yanagawa Yuki
Department Of Molecular Cellular And Developmental Biology Yale University
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ITO Naoko
Department of Exercise and Health Sciences, Faculty of Education, Yamaguchi University
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Sato Takako
The Deepstar Group Japan Marine Science And Technology Center
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MIYAWAKI Miyuki
Department of Bioproduction Science, Faculty of Horticulture, Chiba University
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AITO Misako
Department of Bioproduction Science, Faculty of Horticulture, Chiba University
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KENDRICK Richard
Laboratory for Signal Transduction and Photoperception, Frontier Research Program, The Institute of
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Nakagawa H
Graduate School Of Science And Technology Chiba University
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Nakagawa Hiroki
Fac.hort Chiba Univ.
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Nakagawa Hiroki
Department Of Agricultural Chemistry Faculty Of Agriculture Saga University
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Sato T
Institute Of Applied Biochemistry University Of Tsukuba
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Ito N
Tokyo Gakugei Univ. Tokyo Jpn
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Nakagawa Hiroki
Dep. Of Applied Biochemistry And Food Sciences Fac. Of Agriculture Saga Univ.
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Tanaka Kiyoshi
Coll. Environ. Health Azabu Univ.
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Aito Misako
Department Of Bioproduction Science Faculty Of Horticulture Chiba University
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Ito Naoko
Department Of Critical Care Medicine Iwate Medical University School Of Medicine
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Tanaka Keiji
Division Of Ccu Nippon Medical School
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Sato T
Iwate Biotechnology Research Center:department Of Biotechnology And Environmental Chemistry National
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Tanaka Keiji
Division Of Cardiology Department Of Internal Medicine Nippon Medical School
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Ito Naoko
Department Of Bioproduction Science Faculty Of Horticulture Chiba University:laboratory For Signal T
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Miyawaki Miyuki
Department Of Bioproduction Science Faculty Of Horticulture Chiba University
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Sato Tsutomu
Graduate School Of Science And Technology Faculty Of Agriculture Niigata University
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Tanaka Kyoko
Graduate School of Life and Environmental Sciences, Kyoto Prefectural University
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KENDRICK Richard
Laboratory for Signal Transduction and Photoperception, Frontier Research Program, The Institute of Physical and Chemical Research (Riken)
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