Characterization of glycoprotein obtained from the skin mucus of an Antarctic fish, Trematomus bernacchii
スポンサーリンク
概要
- 論文の詳細を見る
The epithelial secretions were collected by scraping the skin of Trematomus bernacchii and seven species of temperate-water fishes and examined for the mucous glycoproteins by gel filtration on a Sepharose CL-4B column. In most cases, the glycoproteins were excluded at the void volume and separated from other mucous constitutents. The yields of the glycoproteins resolved were usually high (35-45%) in the following fishes secreting large quantities of thick mucus from their epidermis: Callionymus lunatus, Misgurnus anguillicaudatus, Anguilla japonica and Leiognathus nuchalis. A fish with hard scales, Carassius auratus, gave a mucous glycoprotein in a low yield (8.4%). Similarly, the glycoproteins of T. bernacchii, Trachurus japonicus and Acanthogobius fiavimanus were obtained in their respective yields of 17.5%, 18.9%, and 14.2%. The carbohydrate composition of the mucous glycoprotein in T. bernacchii was determined to consist of N-acetylneuraminic acid, N-acetylgalactosamine, galactose, mannose, fucose and glucose by analytical methods including thin-layer chromatography, high-performance liquid chromatography and gas-liquid chromatography. The molar ratio of N-acetylneuraminic acid, N-acetylgalactosamine and galactose (1.0:4.8:1.2) suggests that major carbohydrate chains are constituted by such mono- and disaccharide units as JV-acetylgalactosamine, N-acetylneuraminyl-N-acetylgalactosamine, and galactosyl-N-acetylgalactosamine. These carbohydrate units may link through O-glycosidic linkage to threonine and serine residues in the core polypeptide. Assuming such a structure of the carbohydrate unit, it becomes probable that the mucous glycoprotein secreted from T. bernacchii epithelial tissue has a similar ability to depress freezing point for the antifreeze glycoproteins present in the serum.
- 国立極地研究所の論文
著者
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Fukuchi Mitsuo
National Institute of Polar Research, 10-3 Midori-cho, Tachikawa-shi, Tokyo 190-8518, Japan
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Nakagawa Hiroki
Department of Agricultural Chemistry, Faculty of Horticulture, Chiba University
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Fukuda Yasushi
Faculty Of Education Kumamoto University
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Nakagawa Hiroki
Department Of Agricultural Chemistry Faculty Of Agriculture Saga University
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Asakawa Makio
Faculty Of Education Kumamoto University
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Fukuchi Mitsuo
National Inst. Of Polar Res. Kaga Tokyo 173-8515 Jpn
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Fukuchi Mitsuo
National Inst. Of Polar Res. 10-3 Midori-cho Tachikawa Tokyo 190-8518 Jpn
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- ACKNOWLEDGMENTS
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- PREFACE
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