Purification and Characterization of Glycoprotein from the Skin Mucus of the Rainbow Trout, Salmo gairdneri

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概要

• 論文の詳細を見る

• Mucus glycoprotein (RGP) was purified and characterized from the skin mucus of rainbow trout, Salmo gairdneri. RGP was found to contain 30.1% NeuAc, 26.0% GalNAc, 5.0% Gal, and 26.0% amino acids. The protein moiety of RGP is very rich in Thr (32.4 mol%). Neither NeuGc nor KDN (2-keto-3-deoxy-D-glycero-D-galacto-nononic acid) was found in RGP. Alkaline borohydride treatment of RGP yielded a major disaccharide alditol, NeuAcx2→6GalNAc-ol and more than 4 minor oligosaccharide alditols including NeuAc→(GalNAcx1→)GalNAc-ol. It was evident that an average RGP molecule has approximately 500 NeuAc-containing oligosaccharide chains, which are attached to the Thr and Ser residues of the protein moiety and spaced at an average of 3 amino acids apart.

• 社団法人日本農芸化学会の論文
• 1997-04-23

著者

• Nakagawa Hiroki

  Department of Agricultural Chemistry, Faculty of Horticulture, Chiba University

• Nakagawa H

  Graduate School Of Science And Technology Chiba University

• Nakagawa Hiroki

  Fac.hort Chiba Univ.

• Nakagawa Hiroki

  Department Of Agricultural Chemistry Faculty Of Agriculture Saga University

• HAMA Yoichiro

  Department of Applied Biological Sciences, Faculty of Agriculture, Saga University

• Nakagawa Hiroki

  Dep. Of Applied Biochemistry And Food Sciences Fac. Of Agriculture Saga Univ.

• Hama Y

  Applied Biological Science Saga University

• Sumi T

  Dep. Of Bioengineering Kumamoto National Coll. Of Technol.

• SUMI Toshihisa

  Department of Applied Biological Sciences, Faculty of Agriculture, Saga University

• ASAKAWA Makio

  Laboratory of Food Science, Faculty of Education, Kumamoto University

• Asakawa M

  Laboratory Of Food Science Faculty Of Education Kumamoto University

• Asakawa Makio

  Laboratory Of Food Science Faculty Of Education Kumamoto University

• MARUYAMA Daisuke

  Department of Cell Biology, Faculty of Science, Saitama University

• Hama Y

  Department Of Applied Biochemistry And Food Sciences Faculty Of Agriculture Saga University

• Hama Yoichiro

  Department Of Applied Biochemistry And Food Science Saga University

• Maruyama Daisuke

  Department Of Applied Biological Sciences Faculty Of Agriculture Saga University

• MARUYAMA Daisuke

  Department of Anesthesiology, Sapporo Medical University School of Medicine

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