Purification and Characterization of α-Mannosidase and β-Ν-Acetylhexosaminidase from Watermelon Fruit (Biological Chemistry)
スポンサーリンク
概要
- 論文の詳細を見る
Watermelon fruit was found to be rich in α-mannosidase (EC 3.2.1.24) and β-N-acetylhexosaminidase (EC 3.2.1.52). A simple procedure has been devised for the simultaneous isolation of α-mannosidase and β-N-acetylhexosaminidase from watermelon fruit. The purified enzymes were electrophoretically homogeneous and sufficiently free from other glycosidases. The molocular weights of α-mannosidase and β-N-acetylhexosaminidase were estimated to be 270,000 and 150,000 by Sephadex G-200 gel filtration. The enzymes had their maximum activities at pHs 4.0〜4.5 and 5.0〜5.5, and their Km values for the corresponding p-nitrophenyl glycoside were 2.3 and 0.65 mM, respectively. α-Mannosidase required Zn^<2+> to retain its activity and liberated 60% of the mannose from ovalbumin glycopeptide.
- 社団法人日本農芸化学会の論文
- 1988-09-23
著者
-
Nakagawa Hiroki
Department of Agricultural Chemistry, Faculty of Horticulture, Chiba University
-
Nakagawa Hiroki
Department Of Agricultural Chemistry Faculty Of Agriculture Saga University
-
Uda Yutaka
Laboratory Of Health Chemistry Niigata College Of Pharmacy
-
ASAKAWA Makio
Laboratory of Food Science, Faculty of Education, Kumamoto University
-
Asakawa Makio
Laboratory Of Food Science Faculty Of Education Kumamoto University
-
ENOMOTO Noriyuki
Department of Agricultural Chemistry, Faculty of Agriculture, Saga University
-
Enomoto Noriyuki
Department Of Agricultural Chemistry Faculty Of Agriculture Saga University
関連論文
- A Nitrate Reductase Inactivator Protein from Spinach. Purification, Molecular Weight and Subunit Composition
- Inhibition of Proteasome by MG-132 Treatment Causes Extra Phragmoplast Formation and Cortical Microtubule Disorganization during M/G_1 Transition in Synchronized Tobacco Cells
- Overexpression of a gene for 26S proteasome subunit RPN10 confers enhanced resistance to canavanine, an analog of arginine, in transgenic rice (Oryza sativa L.)
- Cell-cycle-related variation in proteins in suspension-cultured rice cells
- Cell-Cycle Dependent Dynamic Change of 26S Proteasome Distribution in Tobacco BY-2 Cells
- Cloning and Sequencing of cDNA from Oryza sativa encoding a homolog to non-ATPase subunit,MBP1, of 26S Proteasome in Arabidopsis thaliana
- Changes in Proteasome Levels in Spinach (Spinacia oleracea) Seeds during Imbibition and Germination
- Immuno-gold Localization of Nitrate Reductase in Spinach (Spinacia oleracea) Leaves
- Isolation and Some Properties of a 115-Kilodalton Nitrate Reductase-Inactivator Protein from Spinacia oleracea
- Control by Glutamine of the Synthesis of Nitrate Reductase in Cultured Spinach Cells : PROTEINS, ENZYMES AND METABOLISM
- Size and Levels of mRNA for Acid Invertase in Ripe Tomato Fruit
- Immunological Characterization of Nitrate Reductase in Different Tissues of Spinach Seedlings
- Polygalacturonase mRNA of Tomato : Size and Content in Ripe Fruits
- Cell-Free Synthesis of a Putative Precursor of Polygalacturonase in Tomato Fruits
- Effect of Heat Treatment on the Development of Polygalacturonase Activity in Tomato Fruit during Ripening
- Monoclonal Antibodies Spicific for NADH-Ferricyanide Reductase Domain of Spinach Leaf Nitrate Reductase
- Isoforms of Invertase in Grape Berries(Biological Chemistry)
- Involvement of Proteasome-Ubiquitin System in Wound-Signaling in Tobacco Plants
- Quantitative Anhydrous Mercaptolysis of Algal Galactans Followed by HPLC of Component Sugars
- Purification and Characterization of the Alkaline Serine Protease Produced by Bacillus sp.N4 Strain from Fish Skin Mucus
- Effect of flunitrazepam on sleep and memory
- Alcohol effect on sleep electroencephalography by fast Fourier transformation
- Sequence Analysis of Cloned cDNA and Proteolytic Fragments for Nitrate Reductase from Spinacia oleracea L.
- Chlorate Reducing Activity of Spinach Nitrate Reductase
- Characterization of glycoprotein obtained from the skin mucus of an Antarctic fish, Trematomus bernacchii
- Purification and Characterization of Glycoprotein from the Skin Mucus of the Rainbow Trout, Salmo gairdneri
- Structural Characterization of the Carbohydrate Chains from the Skin Mucus Sialoglycoprotein of the Stingray Dasyatis akajei
- Isolation and Properties of a Sialoglycoprotein from the Skin Mucus of the Stingray Dasyatis akajei
- Effects of Inorganic Anions on the Activation of Acid Sialidases
- Purification and Characterization of α-Mannosidase and β-Ν-Acetylhexosaminidase from Watermelon Fruit (Biological Chemistry)
- Selective Hydrolysis of the 3,6-Anhydrogalacotosidic Linkage in Red Algal Galactan : A Combination of Reductive Acid Hydrolysis and Anhydrous Mercaptolysis
- Effects of Phthalate Esters on Liver Lysosomal Acid Lipase and Acid Esterase in Vitro
- A Novel Sol-Gel Approach to Highly Condensed Silicas at Low Temperature
- Hepatic Triglyceride Accumulation as an Index of the Bioavailability of Oxidized Methionine to the Growing Rat
- Immunological Properties of Two Acid Phosphatases from Tomato Fruit
- Effect of Heat Treatment on Changes in Acid Phosphatase Activity during Tomato Ripening
- Identification of Possible Intermediates in in Vivo Degradation of Spinach Nitrate Reductase