Monoclonal Antibodies Spicific for NADH-Ferricyanide Reductase Domain of Spinach Leaf Nitrate Reductase

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概要

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• Two monoclonal antibodies, 17(3)9 and 36(79)4, were prepared against nitrate reductase from Spinacia oleracea L. leaves. An enzyme-linked immunosorbent assay showed that 17(3)9, but not 36(79)4, reacted more strongly to heat-denatured than native antigen. These antibodies inhibited NADH-nitrate reductase as well as its various partial activities including reduced methyl vilogen-nitrate reductase, reduced flavin mononucleotide-nitrate reductase and NADH-cytochrome c reductase activities, but not NADH-ferricyanide reductase activity. Immunoblotting after electrophoretic separation of nitrate reductase fragments obtained by Staphyrococcus aureus V8 protease digestion of native enzyme revealed that the two monoclonal antibodies bind to different epitopes located on the 28 kDa of the NADH-ferricyanide reductase domain.

• 日本植物生理学会の論文

著者

• IKEDA Tadashi

  Department of Surgery, Keio University School of Medicine

• Sueyoshi Kuni

  Graduate School Of Science And Technology ; Niigata University

• Ogura Nagao

  Department of Agricultural Chemistry, Faculty of Horticulture, Chiba University

• Nakagawa Hiroki

  Department of Agricultural Chemistry, Faculty of Horticulture, Chiba University

• Ogura N

  Division Of Bioproduction Science Faculty Of Horticulture Chiba University

• Ogura N

  Chiba Univ. Chiba Jpn

• Ogura Nagao

  Department Of Agricultural Chemistry Faculty Of Horticulture Chiba University

• Ikeda T

  Kyoto Univ. Kyoto Jpn

• SUEYOSHI Kuni

  Department of Applied Biological Chemistry, Faculty of Agriculture, Niigata University

• Ochiai Kuniyasu

  Department of Microbiology, Nihon University School of Dentistry, Japan

• Ikeda Tadashi

  Department Of Cardiovascular Surgery Kyoto University Graduate School Of Medicine

• Nakagawa H

  Graduate School Of Science And Technology Chiba University

• Nakagawa Hiroki

  Fac.hort Chiba Univ.

• Nakagawa Hiroki

  Department Of Agricultural Chemistry Faculty Of Agriculture Saga University

• Yamagishi Kenji

  Department of Agricultural Chemistry, Faculty of Horticulture, Chiba University

• Fukushima K

  Nihon Univ. School Of Dentistry At Matsudo Matsudo Jpn

• Kubo Yoshihiro

  Department of Agricultural Chemistry, Faculty of Horticulture, Chiba University

• Fukushima Kazuo

  Department of Microbiology, Nihon University School of Dentistry at Matsudo

• Nakagawa Hiroki

  Dep. Of Applied Biochemistry And Food Sciences Fac. Of Agriculture Saga Univ.

• Kubo Y

  Graduate School Of Natural Science And Technology Okayama University

• Sueyoshi Kuni

  Department Of Agricultural Chemistry Faculty Of Horticulture Chiba University

• Sueyoshi Kuni

  Department Of Agricultural Chemistry Faculty Of Agriculture Kobe University

• Nakagawa H

  Fac. Horticulture. Chiba Univ.

• Ogura N

  Department Of Agricultural Chemistry Faculty Of Horticulture Chiba University

• Sueyoshi Kuni

  Department Of Applied Biological Chemistry Faculty Of Agriculture Niigata University

• Yamagishi K

  Tokyo Univ. Agriculture Tokyo Jpn

• Yamagishi Kenji

  National Agricultural Research Center For The Tohoku Region National Agricultural And Bio-oriented R

• Yamagishi Kenji

  Department Of Agricultural Chemistry Faculty Of Horticulture Chiba University

• Ochiai Kuniyasu

  Department Of Microbiology Nihon University School Of Dentistry

• Ochiai Kuniyasu

  Department Of Microbiology Nihon University School Of Dentistry At Matsudo

• Kubo Y

  Chiba Univ. Chiba Jpn

• Fukushima Kazuo

  Department Of Environmental Sciences Faculty Of Science Shinshu University

• Kubo Yoshihiro

  Department Of Agricultural Chemistry Faculty Of Horticulture Chiba University

• Ikeda Tadashi

  Department Of Cardiovascular Surgery Graduate School Of Medicine Kyoto University

• Ochiai Kuniyasu

  Department Of Microbiology Division Of Immunology And Pathobiology Dental Research Center Nihon University School Of Dentistry

• Ochiai Kuniyasu

  Department of Microbiology and Immunology, Nihon University School of Dentistry at Matsudo

• OCHIAI Kuniyasu

  Department of Bacteriology, Nihon University, School of Dentistry

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