Quantitative and Isotopic Analysis of Amino Acids, Allantoin, and Allantoic Acid in Soybeans by LC-MS Using the Atmospheric Pressure Chemical lonization Method(Plant Nutrition)
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概要
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In this report, we determined whether liquid chromatography mass spectrometry (LC-MS) using the atmospheric pressure chemical ionization (APCI) method could be applied for isotopic analysis of the high polar compounds, allantoin, and allantoic acid, which are the major transport forms of N from soybean nodules. The peak areas of the ion chromatogram of authentic allantoin and allantoic acid obtained by selected ion monitoring (SIM) were proportional to the contents of the compounds. The data obtained from the ion intensity using the scan mode and the area of the ion chromatogram using the SIM mode were entirely consistent with the isotope ratio of the compounds. The allantoin and allantoic acid concentrations in the xylem sap of soybeans at various growth stages were measured by the following two methods using LC-MS ; an absolute calibration method and an isotopelabeled internal standard method. The values estimated by the isotope-labeled internal standard method were more consistent with those obtained by the colorimetric method. Furthermore, amino acids could be detected under the same analytical conditions. The ^<15>N ratio of asparagine and aspartic acid in the non-nodulating soybean xylem sap which had been supplied with ^<15>N-labeled nitrate could be measured. These results indicate that LC-MS (APCI) is a suitable method for isotopic analysis of high polar compounds, including allantoin and allantoic acid in plant xylem sap and tissues.
- 社団法人日本土壌肥料学会の論文
著者
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Sueyoshi Kuni
Graduate School Of Science And Technology ; Niigata University
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OHTAKE Norikuni
Graduate School of Science and Technology ; Niigata University
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OHYAMA Takuji
Graduate School of Science and Technology ; Niigata University
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Ohyama Takuji
Faculty of Agriculture, Niigata University
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Ohyama Takuji
Faculty Of Agriculture Niigata University
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OHTAKE Norikuni
Faculty of Agriculture, Niigata University
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SUEYOSHI Kuni
Faculty of Agriculture, Niigata University
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ITO Sayuri
Faculty of Agriculture, Niigata University
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FUJIKAKE Hiroyuki
Graduate School of Science and Technology, Niigata University
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Takano Aya
Faculty of Agriculture, University of Niigata
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Yamazaki Akihiko
Faculty of Agriculture, University of Niigata
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Fujikake Hiroyuki
Faculty of Agriculture, University of Niigata
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Ohtake Norikuni
Dept. Appl. Biol. Chem. Fac. Agric. Niigata Univ.
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Takano Aya
Faculty Of Agriculture University Of Niigata
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Sueyoshi Kuni
Department Of Agricultural Chemistry Faculty Of Horticulture Chiba University
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Yamazaki Akihiko
Graduate School Of Science And Technology Niigata University
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Ito Sayuri
Plant Positron Imaging Group Quantum Beam Science Directorate Japan Atomic Energy Agency
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Fujikake Hiroyuki
Graduate School Of Science And Technology Niigata University
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Sueyoshi Kuni
Department Of Applied Biological Chemistry Faculty Of Agriculture Niigata University
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Ohyama T
Niigata Univ. Niigata Jpn
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