Amino Acid Composition in Xylem Sap of Soybean Related to the Evaluation of N_2 Fixation by the Relative Ureide Method
スポンサーリンク
概要
- 論文の詳細を見る
For the estimation of N_2 fixation of soybean (Glycine max (L.) Merr.) by the relative ureide method, we modified the original equation [ureide-N/(ureide-N+α-amino-N+nitrate-N)] proposed by Herridge et al.to [ureide-N/(ureide-N+2×α-amino-N+nitrate-N)], based on the observation that asparagine which has two N atoms in one molecule was the major amino acid in the xylem sap of soybean plants. However, since the amino acid composition may change with the growth stages or environmental factors such as N availability, it is necessary to determine whether the factor "2×" is appropriate. In the present paper, the experiments were carried out to analyze the levels and relative composition of amino acids and amides in the xylem sap of soybean cv. Enrei which was grown in the field with three fertilizer treatments, i.e.control with a small amount of basal dressing of ammonium sulfate, deep placement or top dressing of coated urea (slow release N fertilizer). Although the composition of the amino acids and amides changed with the fertilizer treatments or growth stages, the major form was always asparagine. The concentration of asparagine as well as glutamine, arginine, and histidine increased with growth till the R5 stage. The levels of valine, lysine, threonine, isoleucine, leucine, phenylalanine, and serine rose remarkably at the maturation stage. In contrast, the levels of glutamic acid, GABA, alanine, and glycine decreased through maturation. In the control treatment, the proportion of asparagine accounted for about 30-40% of total amino-N till the R3 stage, then increased to about 50% at maturation. The proportion of asparagine exceeded 70% at the R5 stage with either deep placement or top dressing of coated urea. Irrespective of fertilizer treatments or growth stages, the average number of N atoms per amino compound was relatively constant, ranging from 1.5 to 1.9 (average 1.7) based on determinations by the amino acid analyzer. In conclusion, it was confirmed that the relative ureide-N values based on the 2×α-amino-N equation are more relevant irrespective of stages and fertilizer treatments than the original equation.
- 社団法人日本土壌肥料学会の論文
著者
-
OHTAKE Norikuni
Graduate School of Science and Technology ; Niigata University
-
Ohyama Takuji
Faculty Of Agriculture Niigata University
-
OHTAKE Norikuni
Faculty of Agriculture, Niigata University
-
TAKAHASHI Yoshihiko
Faculty of Agriculture, Niigata University
-
Ohtake Norikuni
Dept. Appl. Biol. Chem. Fac. Agric. Niigata Univ.
-
Nishiwaki Toshikazu
Faculty of Agriculture, Niigata University
-
Takahashi Yoshihiko
Niigata Agricultural Experiment Station
-
Chinushi Toshikazu
Niigata Agricultural Experiment Station
-
Nishiwaki Toshikazu
Faculty Of Agriculture Niigata University
-
Chinushi Toshiaki
Niigata Agricultural Experiment Station
-
Nakano Tomio
Niigata Agricultural Experiment Station
-
Mizukoshi Kazushi
Faculty of Agriculture, Niigata University
-
Minagawa Ritsuko
Faculty of Agriculture, Niigata University
-
Tamura Y
Faculty Of Agriculture Niigata University
-
Takahashi Y
National Institute Of Agro-environmental Sciences
-
Nakano T
Faculty Of Agriculture Niigata University
-
Mizukoshi Kazushi
Faculty Of Agriculture Niigata University
-
Minagawa Ritsuko
Faculty Of Agriculture Niigata University
関連論文
- Two-component high-affinity nitrate transport system in barley : Membrane localization, protein expression in roots and a direct protein-protein interaction
- Nitrogen Fixation and Translocation in Young Sugarcane (Saccharum officinarum L.) Plants Associated with Endophytic Nitrogen-Fixing Bacteria
- Localization of MT2a Gene Promoter Expression Is Different from the Site(s) of Copper Accumulation in the Roots of Transgenic Arabidopsis thaliana
- Enhancement of the nitrogen fixation efficiency of genetically-engineered Rhizobium with high catalase activity(MICROBIAL PHYSIOLOGY AND BIOTECHNOLOGY)
- Isolation and characterization of a novel Arabidopsis thaliana mutant that shows low nitrate concentration in shoots(Plant Nutrition)
- Absorption and translocation of nitrogen in cucumber (Cucumis sativus L.) plants using the ^N tracer technique(Plant Nutrition)
- Analysis of the nitrogen nutrition of soybean plants with deep placement of coated urea and lime nitrogen(Plant Nutrition)
- Characteristics of initial growth of hypernodulation soybean mutants, NOD1-3, NOD2-4 and NOD3-7, affected by inoculation of bradyrhizobia and nitrate supply(Plant Nutrition)
- Effect of nitrogen supply on nitrogen and carbohydrate constituent accumulation in rhizomes and storage roots of Curcuma alismatifolia Gagnep.(Plant Nutrition)
- Comparison of the depth of placement of lime nitrogen on growth, N_2 fixation activity, seed yield and quality of soybean (Glycine max (L.) Merr.) plants(Plant Nutrition)
- Allocation of photosynthetic products in soybean during the early stages of nodule formation(Plant Nutrition)
- Determination of the uptake and utilization of nitrogen in Curcuma alismatifolia Gagnep. using ^N isotope(Plant Nutrition)
- Long-Term Effect of Nitrate Application from Lower Part of Roots on Nodulation and N_2 Fixation in Upper Part of Roots of Soybean (Glycine max (L.) Merr.) in Two-Layered Pot Experiment(Plant Nutrition)
- ^N Analysis of the Promotive Effect of Deep Placement of Slow-Release N Fertilizers on Growth and Seed Yield of Soybean (Plant Nutrition)
- Effect of Placement of Urea and Coated Urea Fertilizers on Yield and Quality of Soybean (Glycine max (L.) Merr.) Seeds(Plant Nutrition)
- Contribution of Shoots and Roots to In Vivo Nitrate Reduction in NADH-Specific Nitrate Reductase-Deficient Mutant Seedlings of Barley (Hordeum vulgare L.)(Plant Nutrition)
- Quantitative and Isotopic Analysis of Amino Acids, Allantoin, and Allantoic Acid in Soybeans by LC-MS Using the Atmospheric Pressure Chemical lonization Method(Plant Nutrition)
- Systemic and Local Effects of Long-Term Application of Nitrate on Nodule Growth and N_2 Fixation in Soybean (Glycine max [L.] Merr.) (Plant Nutrition)
- Site of Nitrogen Accumulation in Tulip (Tulipa gesneriana L.) Roots during Winter
- Site of Nitrogen Accumulation in Tulip (Tulipa gesneriana L.) Roots during Winter (Plant Nutrition)
- Photoassimilate Partitioning in Hypernodulation Mutant of Soybean (Glycine max (L.) Merr.) NOD1-3 and Its Parent Williams in Relation to Nitrate Inhibition of Nodule Growth (Plant Nutrition)
- Effects of Anion Channel Blockers on Xylem Nitrate Transport in Barley Seedlings
- Effect of Exogenous NH^+_4-N Supply on Distribution of Ureide Content in Various Tissues of Alfalfa Plants, Medicago sativa
- 8-25 Short-term effects of ammonium sulfate application on distribution of ureide and amino acid-N in bleeding sap and underground-parts of alfalfa, Medicago sativa : Effect of exogenous N supply on ureide and amino acid-N
- Rapid and Reversible Nitrate Inhibition of Nodule Growth and N_2 Fixation Activity in Soybean (Glycine max (L.) Merr.)
- Possible Involvement of Photosynthetic Supply in Changes of Nodule Characteristics of Hypernodulating Soybeans
- INACTIVATION OF BARLEY NITRATE REDUCTASE BY 14-3-3 PROTEIN
- Determination of Leghemoglobin Components and Xylem Sap Composition by Capillary Electrophoresis in Hypernodulation Soybean Mutants Cultivated in the Field
- Changes in concentration of leghemoglobin components in hypernodulation mutants of soybean
- Regulation of Accumulation of β-Subunit of β-Conglycinin in Soybean Seeds by Nitrogen
- Real-time imaging of nitrogen fixation in an intact soybean plant with nodules using ^N-labeled nitrogen gas(Plant Nutrition)
- Effect of Exogenous Salicylic Acid Supply on Nodule Formation of Hypernodulating Mutant and Wild Type of Soybean
- Temporary Application of Nitrate to Nitrogen-Deficient Soybean Plants at the Mid-to Late-Stages of Seed Development Increased the Accumulation of the β-Subunit of β-Conglycinin, a Major Seed Storage Protein
- Measurement Method of Gytokinis in Soybean Immature Seed using Fluorescent Derivative and LC/MS System.
- 6 Comparative studies on contribution of roots and shoots to in vivo nitrate reduction in wild type and nitrate reductase-deficient mutant of barely
- Role of Xylem Sap Nitrate in the Regulation of Nitrate Reductase Gene Expression in Leaves of Barley (Hordeurn vulgare L.) Seedlings
- Effect of Short-Term Application of Nitrogen on the Accumulation of β-Subunit of β-Conglycinin in Nitrogen-Starved Soybean(Glycine max L.) Developing Seeds
- REGULATION OF NITRATE REDUCTASE GENE EXPRESSION IN BARLEY : 2. ROLE OF ANION CHANNEL
- REGULATION OF NITRATE REDUCTASE GENE EXPRESSION IN BARLEY : 1. ROLE OF XYLEM SAP NITRATE
- Composition of Storage Carbohydrate in Tubers of Yacon : Polymnia sonchifolia
- Effect of Deep Placement of Calcium Cyanamide, Coated Urea, and Urea on Soybean (Glycine max (L.) Merr.) Seed Yield in Relation to Different Inoculation Methods
- Effect of Deep Placement of Controlled Release Nitrogen Fertilizer (Coated Urea) on Growth, Yield, and Nitrogen Fixation of Soybean Plants
- Nitrate Transport Pathway into Soybean Nodules Traced by Tungstate and ^NO^-_3
- Effect of Placement of Coated Urea Fertilizer on Root Growth and Rubidium Uptake Activity in Soybean Plant
- The Autoregulation of Nodulation Mechanism is Related to Leaf Development
- Effect of basal side-dressing of various types of coated urea fertilizer on shoot growth, yield components and seed composition of soybean (Glycine max (L.) Merr.)(Soil Chemistry and Mineralogy)
- Amino Acid Composition in Xylem Sap of Soybean Related to the Evaluation of N_2 Fixation by the Relative Ureide Method
- Lack of β-Subunit of β-Conglycinin in Non-Nodulating Isolines of Soybean
- Evaluation of N_2 Fixation and N Absorption Activity by Relative Ureide Method in Field-Grown Soybean Plants with Deep Placement of Coated Urea
- NITROGEN ASSIMILATION IN SOYBEAN NODULES : I. The Role of GS/GOGAT System in the Assimilation of Ammonia Produced by N_2-fixation
- NITROGEN ASSIMILATION IN SOYBEAN NODULES : III.Effects of Rhizosphere pO_2 on the Assimilation of ^N_2 in Nodules Attached to Intact Plants
- NITROGEN ASSIMILATION IN SOYBEAN NODULES : II. ^N_2 Assimilation in Bacteroid and Cytosol Fractions of Soybean Nodules
- REGULATION OF β-CONGLYCININ ACCUMULATION IN SOYBEAN SEEDS BY NITROGEN DEFICIENCY AND HORMONE LEVELS
- ASSIMILATION AND TRANSPORT OF NITROGENOUS COMPOUNDS ORIGINATED FROM ^N_2 FIXATION AND ^NO_3 ABSORPTION
- Assimilation and Transport of Fixed Nitrogen in Soybean Nodule as Revealed with 15N
- Quantitative analysis of the initial transport of fixed nitrogen in nodulated soybean plants using ^N as a tracer(Plant nutrition)