Enhancement of the nitrogen fixation efficiency of genetically-engineered Rhizobium with high catalase activity(MICROBIAL PHYSIOLOGY AND BIOTECHNOLOGY)

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概要

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• The vktA catalase gene, which had been cloned from Vibrio rumoiensis S-1^T having extraordinarily high catalase activity, was introduced into the root nodule bacterium, Rhizobium leguminosarum bv. phaseoli USDA 2676. The catalase activity of the vktA-transformed R. leguminosarum cells (free-living) was three orders in magnitude higher than that of the parent cells and this transformant could grow in a higher concentration of exogenous hydrogen peroxide (H_2O_2). The vktA-transformant was inoculated to the host plant (Phaseolus vulgaris L.) and the nodulation efficiency was evaluated. The results showed that the nitrogen-fixing activity of nodules was increased 1.7 to 2.3 times as compared to the parent. The levels of H_2O_2 in nodules formed by the vktA-transformant were decreased by around 73%, while those of leghemoglobins (Lba and Lbb) were increased by 1.2 (Lba) and 2.1 (Lbb) times compared with the parent. These results indicated that the increase of catalase activity in rhizobia could be useful to improve the nitrogen-fixing efficiency of nodules by the reduction of H_2O_2 content concomitantly with the enhancement of leghemoglobins contents.

著者

• Yumoto Isao

  National Institute Of Advanced Industrial Science And Technology

• Morishita Nozomu

  Res. Inst. Of Genome-based Biofactory National Inst. Of Advanced Industrial Sci. And Technol. (aist)

• WEI Min

  Department of Pathology, Osaka City University Medical School

• Yumoto Isao

  Research Institute Of Genome-based Biofactory National Institute Of Advanced Industrial Science And

• Okuyama Hidetoshi

  Hokkaido Univ. Sapporo Jpn

• Ohyama Takuji

  Faculty of Agriculture, Niigata University

• Orikasa Yoshitake

  Department of Food Science, Obihiro University of Agriculture and Veterinary Medicine

• Nodasaka Yoshinobu

  Graduate school of Dental Medicine, Hokkaido University

• Okuyama Hidetoshi

  Laboratory of Environmental Molecular Biology, Graduate School of Environmental Earth Science, Hokka

• Ichise Nobutoshi

  Laboratory of Environmental Molecular Biology, Graduate School of Environmental Earth Science, Hokka

• Morita Naoki

  National Institute of Advanced Industrial Science and Technology (AIST)

• Ohwada Takuji

  Department of Food Science, Obihiro University of Agriculture and Veterinary Medicine

• Okuyama Hidetoshi

  Laboratory Of Environmental Molecular Biology Graduate School Of Environmental Earth Science Hokkaid

• Okuyama Hidetoshi

  Lab. Of Environmental Molecular Biology Graduate School Of Environmental Earth Sci. Hokkaido Univ. S

• Yumoto Isao

  National Institute Of Advanced Industrial Science And Technology (aist)

• Okuyama H

  Hokkaido Univ. Sapporo Jpn

• Morishita Nozomu

  Research Institute Of Genome-based Biofactory National Institute Of Advanced Industrial Science And

• Ohyama Takuji

  Fac. Of Agriculture Niigata Univ. Niigata 950-2181 Jpn

• Ohyama Takuji

  Faculty Of Agriculture Niigata University

• Ohwada Takuji

  Department Of Agricultural And Life Sciences Obihiro University Of Agriculture And Veterinary Medici

• Wei Min

  Department Of Food Science Obihiro University Of Agriculture And Veterinary Medicine

• Ichise Nobutoshi

  Laboratory Of Environmental Molecular Biology Graduate School Of Environmental Earth Science Hokkaid

• Orikasa Yoshitake

  Department Of Food Science Obihiro University Of Agriculture And Veterinary Medicine

• Nodasaka Yoshinobu

  Graduate School Of Dental Medcine Hokkaido University

• YUMOTO Isao

  Hokkaido National Industrial Research Institute

• Yumoto I

  National Institute Of Advanced Industrial Science And Technology (aist)

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