The cell membrane-shielding function of eicosapentaenoic acid for Escherichia coli against exogenously added hydrogen peroxide.
スポンサーリンク
概要
- 論文の詳細を見る
The colony-forming ability of catalase-deficient Escherichia coli mutant genetically modified to produce eicosapentaenoic acid (EPA) showed less decrease than in a control strain producing no EPA, when treated with 0.3 mM hydrogen peroxide (H2O2) under non-growth conditions. H2O2-induced protein carbonylation was enhanced in cells lacking EPA. The amount of fatty acids was decreased more significantly for cells lacking EPA than for those producing EPA. Much lower intracellular concentrations of H2O2 were detected for cells with EPA than those lacking EPA. These results suggest that cellular EPA can directly protect cells against oxidative damage by shielding the entry of exogenously added H2O2.
- Elsevier B.V.の論文
- 2006-12-11
著者
-
Okuyama Hidetoshi
Hokkaido Univ. Sapporo Jpn
-
Okuyama Hidetoshi
Laboratory Of Environmental Molecular Biology Graduate School Of Environmental Earth Science Hokkaid
-
Okuyama Hidetoshi
Lab. Of Environmental Molecular Biology Graduate School Of Environmental Earth Sci. Hokkaido Univ. S
-
Okuyama H
Hokkaido Univ. Sapporo Jpn
-
Orikasa Yoshitake
Department Of Food Science Obihiro University Of Agriculture And Veterinary Medicine
関連論文
- Possible Biosynthetic Pathways for all cis-3,6,9,12,15,19,22,25,28-Hentriacontanonaene in Bacteria
- Enhancement of the nitrogen fixation efficiency of genetically-engineered Rhizobium with high catalase activity(MICROBIAL PHYSIOLOGY AND BIOTECHNOLOGY)
- pfaB products determine the molecular species produced in bacterial polyunsaturated fatty acid biosynthesis
- H_2O_2 Tolerance of Vibrio rumoiensis S-1^T is Attributable to the Cellular Catalase Activity(MICROBIAL PHYSIOLOGY AND BIOTECHNOLOGY)
- H_2O_2 Tolerance of Vibrio rumoiensis S-1^T is Attributable to the Cellular Catalase Activity
- Enhanced heterologous production of eicosapentaenoic acid in Escherichia coli cells that co-express eicosapentaenoic acid biosynthesis pfa genes and foreign DNA fragments including a high-performance catalase gene, vktA.
- Characterization of the Gene Encoding the β-Lactamase of the Psychrophilic Marine Bacterium Moritella marina Strain MP-1
- Gene Cloning and Expression of the Catalase from the Hydrogen Peroxide-Resistant Bacterium Vibrio rumoiensis S-1 and Its Subcellular Localization
- GENE CLONING OF THE FATTY ACID SYNTHASE FROM THE DHA PRODUCING PSYCHROPHILIC BACTERIUM, Vibrio marinus strain MP-1
- Isolation of Vibrio sp.S-1 Exhibiting Extraordinarily High Catalase Activity
- Isolation and characterization of bacteria from soil contaminated with diesel oil and the possible use of these in autochthonous bioaugmentation
- Degradation of long-chain n-alkanes (C36 and C40) by Pseudomonas aeruginosa strain WatG
- Verification of Degradation of n-Alkanes in Diesel Oil by Pseudomonas aeruginosa Strain WatG in Soil Microcosms
- Extremely psychrophilic microalgae isolated from the Antarctic ocean
- The antioxidative function of eicosapentaenoic acid in a marine bacterium, Shewanella marinintestina IK-1
- Bacterial Genes Responsible for the Biosynthesis of Eicosapentaenoic and Docosahexaenoic Acids and Their Heterologous Expression
- Recombinant production of docosahexaenoic acid in a polyketide biosynthesis mode in Escherichia coli.
- A phosphopantetheinyl transferase gene essential for biosynthesis of n-3 polyunsaturated fatty acids from Moritella marina strain MP-1
- An EntD-like phosphopantetheinyl transferase gene from Photobacterium profundum SS9 complements pfa genes of Moritella marina strain MP-1 involved in biosynthesis of docosahexaenoic acid
- GPI-ANCHORED PROTEINS OF PLANT CELLS
- IDENTIFICATION AND CHARACTERIZATION OF A 9-CIS-HEXADECENOIC ACID CIS-TRANS ISOMERASE FROM A PSYCHROTROPHIC BACTERIUM, PSEUDOMONAS SP. STRAIN E-3 (18th Symposium on Polar Biology)
- DISTRIBUTION AND ACCUMULATION OF PHOSPHATASE AND PLASMA MEMBRANE PROTON-ATPase INDUCIBLY SYNTHESIZED IN Spirodela oligorrhiza GROWN UNDER PHOSPHATE-DEFICIENT CONDITIONS
- Significance of Antioxidative Function of Eicosapentaenoic and Docosahexaenoic Acids in Marine Microorganisms
- In vivo conversion of triacylglycerol to docosahexaenoic acid-containing phospholipids in a thraustochytrid-like microorganism, strain 12B
- The cell membrane-shielding function of eicosapentaenoic acid for Escherichia coli against exogenously added hydrogen peroxide.
- Escherichia coli engineered to produce eicosapentaenoic acid becomes resistant against oxidative damages
- Isolation and Characterization of a Novel Thraustochytrid-like Microorganism that Efficiently Produces Docosahexaenoic Acid
- The Escherichia coli highly expressed entD gene complements the pfaE deficiency in a pfa gene clone responsible for the biosynthesis of long-chain n-3 polyunsaturated fatty acids
- Membrane eicosapentaenoic acid is involved in the hydrophobicity of bacterial cells and affects the entry of hydrophilic and hydrophobic compounds
- Baking Properties of Saccharomyces cerevisiae Strains Derived from Brem, a Traditional Rice Wine from Bali
- Efficient Production of Ethanol from Saccharified Crops Mixed with Cheese Whey by the Flex Yeast Kluyveromyces marxianus KD-15