Recombinant production of docosahexaenoic acid in a polyketide biosynthesis mode in Escherichia coli.

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概要

• 論文の詳細を見る

• The docosahexaenoic acid (DHA) biosynthesis gene cluster (pDHA3) from the DHA-producing Moritella marina strain MP-1 includes the genes pfaA, pfaB, pfaC, and pfaD, which are similar to the genes of polyketide biosynthesis. When this cluster was co-expressed in Escherichia coli with M. marina MP-1 pfaE, which encodes phosphopantetheinyl transferase, DHA was biosynthesized. The maximum production of DHA (5% of total fatty acids) was observed at 15°C. This is the first report of the recombinant production of DHA in a polyketide biosynthesis mode.

• Springerの論文

著者

• Morishita Nozomu

  Res. Inst. Of Genome-based Biofactory National Inst. Of Advanced Industrial Sci. And Technol. (aist)

• Morita Naoki

  National Institute of Advanced Industrial Science and Technology (AIST)

• Okuyama Hidetoshi

  Laboratory Of Environmental Molecular Biology Graduate School Of Environmental Earth Science Hokkaid

• Okuyama Hidetoshi

  Lab. Of Environmental Molecular Biology Graduate School Of Environmental Earth Sci. Hokkaido Univ. S

• Okuyama H

  Hokkaido Univ. Sapporo Jpn

• Morishita Nozomu

  Research Institute Of Genome-based Biofactory National Institute Of Advanced Industrial Science And

• 湯 玲子

  (元)パレス化学:(現)三菱化学

• Orikasa Yoshitake

  Department Of Food Science Obihiro University Of Agriculture And Veterinary Medicine

• 湯 玲子

  相模中研

• 湯 玲子

  植工研

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