pfaB products determine the molecular species produced in bacterial polyunsaturated fatty acid biosynthesis

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概要

• 論文の詳細を見る

• When pDHA4, a vector carrying all five pfaA-pfaE genes responsible for docosahexaenoic acid (DHA; 22:6) biosynthesis in Moritella marina MP-1, was coexpressed in Escherichia coli with the individual pfaA-pfaD genes for eicosapentaenoic acid (EPA; 20:5) biosynthesis from Shewanella pneumatophori SCRC-2738, both polyunsaturated fatty acids were synthesized only in the recombinant carrying pfaB for EPA synthesis. Escherichia coli coexpressing a deleted construct comprising pfaA, pfaC, pfaD and pfaE for EPA and pfaB for DHA produced EPA and DHA. Both EPA and DHA were detected in bacteria that inherently contained pfa genes for DHA. These results suggest that PfaB is the key enzyme determining the final product in EPA or DHA biosynthesis.

• Blackwell Publishingの論文

著者

• Morishita Nozomu

  Res. Inst. Of Genome-based Biofactory National Inst. Of Advanced Industrial Sci. And Technol. (aist)

• Okuyama Hidetoshi

  Hokkaido Univ. Sapporo Jpn

• Morita Naoki

  National Institute of Advanced Industrial Science and Technology (AIST)

• Okuyama Hidetoshi

  Laboratory Of Environmental Molecular Biology Graduate School Of Environmental Earth Science Hokkaid

• Okuyama Hidetoshi

  Lab. Of Environmental Molecular Biology Graduate School Of Environmental Earth Sci. Hokkaido Univ. S

• Okuyama H

  Hokkaido Univ. Sapporo Jpn

• Morishita Nozomu

  Research Institute Of Genome-based Biofactory National Institute Of Advanced Industrial Science And

• Tanaka Miyuki

  Department Of Home Economics Aichi University Of Education

• 湯 玲子

  (元)パレス化学:(現)三菱化学

• Orikasa Yoshitake

  Department Of Food Science Obihiro University Of Agriculture And Veterinary Medicine

• 湯 玲子

  相模中研

• 湯 玲子

  植工研

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