Isolation of Vibrio sp.S-1 Exhibiting Extraordinarily High Catalase Activity

概要

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A bacterium exhibiting extraordinarily high catalase activity was isolated from a drain pool of a Ash product Processing plant that uses H_2O_2 as a bleaching and microbicidal agent. The isolate was a gram-negative, rodshped, oxidase-positive, facultative aerobe capable of both fermentative and respiratory metabolism, and sensitive to vibriostatic compound O/129. Although the isolate was non-flagelltcd, its taxonomic chracteristics and phylogenetic position based on a 16S rRNA analysis indicated that it belonged to the genus Vibrio. A culture medium containing living cells and a cell-free extract prepared from 48-A cultured cells of the isolate exhibited catalase activity of 680 units/mg cells and 7276 units/mg protein, respectively. The activity of the cell-free extract of the isolate was 2 orders greater than those of Escherichia coli and Bacillus subtiliss. Application of the newly isolated bacterium or its enzyme will provide an effective means of decomposing H_2O_2 contained in industrial waste such as that produced in the paper, food, textile, and semiconductor industries.
社団法人日本生物工学会の論文
1998-01-25