A phosphopantetheinyl transferase gene essential for biosynthesis of n-3 polyunsaturated fatty acids from Moritella marina strain MP-1
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概要
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A phosphopantetheinyl transferase (PPTase) gene (pfaE), cloned from the docosahexaenoic acid (DHA)-producing bacterium Moritella marina strain MP-1, has an open reading frame of 861 bp encoding a 287-amino acid protein. When the pfaE gene was expressed with pfaA–D, which are four out of five essential genes for biosynthesis of eicosapentaenoic acid (EPA) derived from Shewanella pneumatophori SCRC-2738 in Escherichia coli, the recombinant produced 12% EPA of total fatty acids. This suggests that pfaE encodes a PPTase required for producing n−3 polyunsaturated fatty acids, which is probably involved in the synthesis of DHA in M. marina strain MP-1.
- Elsevier B.V.の論文
- 2006-08-07
著者
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Morishita Nozomu
Res. Inst. Of Genome-based Biofactory National Inst. Of Advanced Industrial Sci. And Technol. (aist)
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Okuyama Hidetoshi
Hokkaido Univ. Sapporo Jpn
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Morita Naoki
National Institute of Advanced Industrial Science and Technology (AIST)
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Okuyama Hidetoshi
Laboratory Of Environmental Molecular Biology Graduate School Of Environmental Earth Science Hokkaid
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Okuyama Hidetoshi
Lab. Of Environmental Molecular Biology Graduate School Of Environmental Earth Sci. Hokkaido Univ. S
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Okuyama H
Hokkaido Univ. Sapporo Jpn
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Morishita Nozomu
Research Institute Of Genome-based Biofactory National Institute Of Advanced Industrial Science And
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Orikasa Yoshitake
Department Of Food Science Obihiro University Of Agriculture And Veterinary Medicine
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