Gene Cloning and Expression of the Catalase from the Hydrogen Peroxide-Resistant Bacterium Vibrio rumoiensis S-1 and Its Subcellular Localization
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概要
- 論文の詳細を見る
The catalase gene, vktA, of the hydrogen peroxide-resistant bacterium Vibrio rumoiensis S-1 has been isolated and sequenced. Plasmid pBSsa1 was obtained by genome library screening and it complemented a catalase-less mutant, Esherichia coli strain UM2, indicating that pBSsa1 contained the catalase gene(vktA). The vktA gene consisted of an open reading frame of 1530 bp encoding a 508 amino-acid protein with a calculated size of 57657.79 Da. The deduced amino acid sequence showed high homology with that of bacterial group III catalases. Investigation of the subcellular catalase localization using an immuno-electron microscopical technique revealed that a larger amount of VktA catalase is present in the periplasmic space than in the cytoplasm. The periplasmic space of this bacterium can therefore be regarded as a reservoir of VktA catalase in V.rumoiensis S-1.
- 社団法人日本生物工学会の論文
- 2000-11-25
著者
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Yumoto Isao
National Institute Of Advanced Industrial Science And Technology
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Morishita Nozomu
Res. Inst. Of Genome-based Biofactory National Inst. Of Advanced Industrial Sci. And Technol. (aist)
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Yumoto Isao
Research Institute Of Genome-based Biofactory National Institute Of Advanced Industrial Science And
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Okuyama Hidetoshi
Hokkaido Univ. Sapporo Jpn
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Okuyama Hidetoshi
Laboratory of Environmental Molecular Biology, Graduate School of Environmental Earth Science, Hokka
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Morita Naoki
National Institute of Advanced Industrial Science and Technology (AIST)
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Okuyama Hidetoshi
Laboratory Of Environmental Molecular Biology Graduate School Of Environmental Earth Science Hokkaid
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Okuyama Hidetoshi
Lab. Of Environmental Molecular Biology Graduate School Of Environmental Earth Sci. Hokkaido Univ. S
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Yumoto Isao
National Institute Of Advanced Industrial Science And Technology (aist)
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Okuyama H
Hokkaido Univ. Sapporo Jpn
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MORITA Naoki
Bioscience and Chemistry Division, Hokkaido National Industrial Research Institute, AIST
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ICHIISE NOBUTOSHI
Laboratory of Environmental Molecular Biology, Graduate School of Environmental Earth Science, Hokka
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MORITA NAOKI
MITI
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KAWASAKI KOSEI
Bioscience and Chemistry Division, Hokkaido National Industrial Research Institute, AIST
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YUMOTO ISAO
MITI
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OKUYAMA HIDETOSHI
Bioscience and Chemistry Division, Hokkaido National Industrial Research Institute, AIST
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YUMOTO ISAO
Bioscience and Chemistry Division, Hokkaodo National Industrial Research Institute
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Morishita Nozomu
Research Institute Of Genome-based Biofactory National Institute Of Advanced Industrial Science And
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Ichise Nobutoshi
Laboratory Of Environmental Molecular Biology Graduate School Of Environmental Earth Science Hokkaid
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YUMOTO Isao
Hokkaido National Industrial Research Institute
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Kawasaki K
Bioscience And Chemistry Division Hokkaido National Industrial Research Institute Aist
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Yumoto I
National Institute Of Advanced Industrial Science And Technology (aist)
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