Production of Two Types of Exopolysaccharide by Novosphingobium rosa

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概要

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• A bacterium, Ikeda-3, that was isolated from the wastewater of a winery was found to produce floating and precipitating exopolysaccharides (EPSs) when grown aerobically in a medium containing sucrose as the sole carbon source. The concentrations of floating and precipitating EPSs were approximately 2.7g/l and 0.6g/l, respectively, in a mini-jar fermentor after incubation of the bacterium at 15℃ for 5d in a synthetic medium containing 1% sucrose, 0.01% CaCO_3, 0.05% MgSO_4, 0.05% K_2HPO_4, 0.0001% Na_2MoO_4, 0.05% peptone and 0.82% bicine. A component analysis of two types of EPS suggested that they represent a novel type of sphingan composed of glucose, rhamnose, mannose and mannuroic acid. Glucuronic acid, which is commonly found in sphingans, was absent. From physiological, biochemical and chemotaxonomic characterization, phylogenetic analysis and DNA-DNA relatedness, Ikeda-3 was identified as Novosphingobium rosa. To our knowledge the production of EPS by N. rosa is reported here for the first time.

• 社団法人日本生物工学会の論文
• 2003-02-25

著者

• Yumoto Isao

  National Institute Of Advanced Industrial Science And Technology

• Yumoto Isao

  Research Institute Of Genome-based Biofactory National Institute Of Advanced Industrial Science And

• MATSUYAMA Hidetoshi

  Department of Bioscience and Technology, School of Engineering, Hokkaido Tokai University

• Yumoto Isao

  National Institute Of Advanced Industrial Science And Technology (aist)

• Minami Hideki

  Department Of Marine Sciences And Technology School Of Engineering Hokkaido Tokai University

• Minami Hideki

  Department Of Marine Sciences And Technology Hokkaido Tokai University

• YUMOTO Isao

  Hokkaido National Industrial Research Institute

• Yumoto I

  National Institute Of Advanced Industrial Science And Technology (aist)

• KAMESAKI TAKUYA

  Department of Bioscience and Technology, Hokkaido Tokai University

• SASAKI RYUICHI

  Department of Bioscience and Technology, Hokkaido Tokai University

• Matsuyama H

  Department Of Bioscience And Technology Hokkaido Tokai University

• Matsuyama Hidetoshi

  Department Of Bioscience And Technology School Of Engineering Hokkaido Tokai University

• Sasaki Ryuichi

  Department Of Bioscience And Technology Hokkaido Tokai University

• Kamesaki Takuya

  Department Of Bioscience And Technology Hokkaido Tokai University

• MATSUYAMA Hidetoshi

  Department of Agricultural Chemistry, The University of Tokyo

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