Identification of a Member of the Serralysin Family Isolated from a Psychrotrophic Bacterium, Pseudomonas fluorescens 114
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概要
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An extracellular metalloprotease named No.114 protease is one of the major secretions of a psychrotrophic bacterium, Pseudomonas fluorescens 114,the cold-adaptation mechanism of which has not been identified. In this study, we purified and cloned No.114 protease, which is a single polypeptide having a molecular mass of 47 kDa. This protease contains a zinc-binding motif (HEXXHXUGUXH : X, arbitrary amino acid ; U, bulky hydrophobic amino acid), glycine-rich repeats (GGXGXD) and no cysteine residue, which are the features specifically found in serralysin subfamily. No.114 protease has its maximum activity at the temperature of 35-40℃, which is about 20℃ lower than that of a serralysin from a mesophilic bacterium, Pseudomonas aeruginosa. All these results imply that No.114 protease from this psychrophilic bacterium is a unique member of the serralysin group characterized by a low optimal temperature.
- 社団法人日本農芸化学会の論文
- 1999-07-23
著者
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MATSUYAMA Hidetoshi
Department of Bioscience and Technology, School of Engineering, Hokkaido Tokai University
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HOSHINO Tamotsu
Hokkaido National Industrial Research Institute
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GODA Takako
Hokkaido National Industrial Research Institute
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OHGIYA Satoru
Hokkaido National Industrial Research Institute
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ISHIZAKI Kozo
Hokkaido National Industrial Research Institute
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Ohgiya Satoru
Hokkaido National Industrial Resaerch Institute
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Goda Takako
Hokkaido National Industrial Research Institute Agency Of Industrial Science And Technology
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KUMETA Hiroyuki
Graduate School of Science and Engineering, Hokkaido Tokai University
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OKAYAMA Tomohiko
Department of Bioscience and Technology, School of Engineering, Hokkaido Tokai University
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SHIMADA Takayuki
Department of Bioscience and Technology, School of Engineering, Hokkaido Tokai University
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Ishizaki K
Hokkaido National Industrial Resaerch Institute
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Hoshino Tamotsu
Bioscience And Chemistry Division Hokkaido National Industrial Research Institute
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Kumeta Hiroyuki
Graduate School Of Science And Engineering Hokkaido Tokai University
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Shimada Takayuki
Department Of Bioscience And Technology School Of Engineering Hokkaido Tokai University
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Okayama Tomohiko
Department Of Bioscience And Technology School Of Engineering Hokkaido Tokai University
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Matsuyama Hidetoshi
Department Of Bioscience And Technology School Of Engineering Hokkaido Tokai University
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Shimada Takayuki
Department of Applied Physics,Faculty of Engineering,University of Tokyo
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Ishizaki Kozo
Hokkaido National Industrial Resaerch Institute
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MATSUYAMA Hidetoshi
Department of Agricultural Chemistry, The University of Tokyo
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