A New n-Alkane Oxidation System from Pseudomonas aeruginosa S7B1
スポンサーリンク
概要
- 論文の詳細を見る
A three-component enzyme system that catalyzes the oxidation of n-hexadecane to n-hexadecanol- 1 has been highly purified from Pseudomonas aeruginosa. The components were tentatively named Protein I, Protein II, and Protein III. Protein I gave an electron paramagnetic resonance signal at g=4.3 which is a characteristic of a nonheme iron protein, and the molecular weight of Protein I was estimated to be about 800, 000 by gel-filtration chromatography. Protein II purified until homogeneous by disc-gel electrophoresis exhibited the spectral characteristics of cytochrome c, and the molecular weight was about 50, 000 by gel-filtration chromatography. Purified Protein III, which gave a single sharp band after disc-gel electrophoresis, exhibited the spectral characteristics of a flavoprotein and had a molecular weight of about 12, 000 by gel-filtration chromatography. All three proteins were shown to be essential for this enzymatic activity in the presence of NADPH and O2.
- 社団法人 日本農芸化学会の論文
著者
-
Minoda Yasuji
Department Of Agricultural Chemistry Faculty Of Agriculture The University Of Tokyo
-
Matsuyama Hidetoshi
Department Of Bioscience And Technology School Of Engineering Hokkaido Tokai University
-
Nakahara Tadaatsu
Institute Of Applied Biochemistry The University Of Tsukuba
関連論文
- キュウリ子葉のクチクラワックスに対するUV-B照射の影響
- Purification and Properties of an Amine Dehydrogenase from Pseudomonas K95 grown on 1,12-Diaminododecane (DAD)(Microbiology & Fermentation Industry)
- Studies on Fermentation Products and Metabolism of Long Chain Alkyl Diamine in Microorganisms(Microbiology & Fermentation Industry)
- A New Metabolic Pathway for meta Ring-fission Compounds of Biphenyl(Biological Chemistry)
- Purification and Some Properties of 2-Hydroxy-6-oxo-6-phenylhexa-2,4-dienoic acid(HOPDA) Reducing Enzyme from Pseudomonas cruciviae S93B1 Involved in the Degradation of Biphenyl(Biological Chemistry)
- Microbial Dehalogenation of Haloalkanes Mediated by Oxygenase or Halidohydrolase
- Microbial Degradation Products from Biphenyl-related Compounds
- Enzymatic Dioxygenation of Biphenyl-2, 3-diol and S-Isopropylcatechol
- Cytochrome c and Bioenergetic Hypothetical Model for Alkaliphilic Bacillus spp.
- Purification and Properties of NAD-Linked 1,2-Butanediol Dehydrogenase from Rhodococcus sp. strain TB-42
- Production of 2-Hydroxybutyric Acid from 1,2-Butanediol by Resting Cells of Rhodococcus sp. Strain TB-42
- Participation of Nitrite Reductase in Conversion of NO_2^- to NO_3^- in a Heterotrophic Nitrifier, Burkholderia cepacia NH-17, with Denitrification Activity
- Cloning and Sequence Analysis of Poly(tetramethylene succinate) Depolymerase from Acidovorax delafieldii Strain BS-3
- Purification and Properties of Culture-Broth-Secreted Esterase from the Polyurethane Degrader Comamonas acidovorans TB-35
- Biodegradation of Dibenzothiophene and 4, 6-Dimethyldiben-zothiophene by Sphingomonas paucimobilis Strain TZS-7
- Decolorization of Molasses Wastewater by Bacillus sp.under Thermophilic and Anaerobic Conditions
- Cloning and Sequence Analysis of a Polyurethane Esterase of Comamonas acidovorans TB-35
- Efficient Conversion of Itaconic Acid to (S)-(+)-Citramalic Acid by Alcaligenes xylosoxydans IL142
- Bioconversion of Maleic Acid to Fumaric Acid by Pseudomonas alcaligenes Strain XD-1
- Production of (S)-(+)-3-Hydroxybutyric Acid from 1,3-Butanediol by Resting Cells of Yeasts
- Determination of the Polyester Polyurethane Breakdown Products and Distribution of the Polyurethane Degrading Enzyme of Comamonas acidovorans Strain TB-35
- Production of Mannosylerythritol Lipids by Candida antarctica from Vegetable Oils(Microbiology & Fermentation Industry)
- Analysis of Dibenzothiophene Metabolic Pathway in Mycobacterium Strain G3
- Analyses of Substrate Specificity of the Desulfurizing Bacterium Mycobacterium sp. G3
- Poly(β-Malic Acid) Production by the Non-Growing Cells of Aureobasidium sp. Strain A-91
- Identification of a Member of the Serralysin Family Isolated from a Psychrotrophic Bacterium, Pseudomonas fluorescens 114
- Purification and Characterization of an Intracellular α-D-Xylosidase from Penicillium wortmannii IFO 7237
- Functions and Potential Applications of Glycolipid Biosurfactants : from Energy-Saving Materials to Gene Delivery Carriers
- Microbial Extracellular Glycolipid Induction of Differentiation and Inhibition of the Protein Kinase C Activity of Human Promyelocytic Leukemia Cell Line HL60
- The Entire Population of Thermus thermophilus Cells Is Always Competent at Any Growth Phase
- Overproduction of Carotenoids in Thermus thermophilus
- Isolation and Partial Characterization of Carotenoid Underproducing and Overproducing Mutants from an Extremetly Thermophilic Thermus thermophilus HB27
- Induction and Characterization of Mutants Enhanced in Assimilability of n-Alkanes in Shake Cultures from a Strain of Candida sp.
- Itaconic Acid Fermentation by a Yeast Belonging to the Genus Candida
- Cell Mass Production of Acinetobacter calcoaceticus in Palm Oil-Limited Chemostant Culture
- Factors Affecting the Production of Succinoyl Trehalose Lipids by Rhodococcus erythropolis SD-74 Grown on n-Alkanes(Microbiology & Fermentation Industry)
- Production of D-Malic Acid from Maleic Acid by Resting Cells of Ustilago sphaerogena Strain S402
- The Effects of Culture Conditions on the Secretion of Human Lysozyme by Saccharomyces cerevisiae A2-1-1A(Biological Chemistry)
- Fermentative Production of Poly(β-L-malic acid), a Polyelectrolytic Biopolyester, by Aureobasidium sp.
- Extracellular Accumulation of the Polyol Lipids, 3,5-Dihydroxydecanoyl and 5-Hydroxy-2-decenoyl Esters of Arabitol and Mannitol, by Aureobasidium sp
- EFFECTS OF UV-B IRRADIATION ON CUTICULAR WAXES IN CUCUMBER COTYLEDONS
- Mode of Action for Mitomycin C Inactivating Factors of Pseudomonas convexa 4-87(Biological Chemistry)
- Partial Purification and Some Properties of Mitomycin C Inactivating Factors of Pseudomonas convexa 4-87(Biological Chemistry)
- Role of Lipase Activators Produced by Saccharomycopsis lipolytica and Calcium Ion in Its Lipase Reaction(Biological Chemistry)
- Isolation and Identification of Lipase Activators from Saccharomycopsis lipolytica(Biological Chemistry)
- Immobilization of an NADH Oxidizing Complex and its Use as a Regenerator of NAD
- Production of Two Types of Exopolysaccharide by Novosphingobium rosa
- Production of a Novel Exopolysaccharide by Rahnella aquatilis
- Antiproliferative Effect of Polyol Lipids, 3,5-Dihydroxydecanoyl and 5-Hydroxy-2-Decenoyl Esters of Arabitol and Mannitol on Lung Cancer Cell Line A549
- Effect if Hydrocarbon Emulsification on Growth and Respiration of Microorganisms in Hydrocarbon Media
- Difference in Degrading p-Nitrophenol between Indigenous Bacteria in a Reactor
- Extracellular Production of a Mannosylerythritol Lipid by a Mutant of Candida sp. from n-Alkanes and Triacylglycerols
- Production of Yeast Cells from Palm Oil at the High Temperature of 40℃
- Extracellular Production of D-(+)-2-Hydroxyglutaric Acid by Yarrowia lipolytica from Glucose under Aerobic, Thiamine-deficient Conditions
- Isocitric Acid Production from n-Alkanes by Candida catenulata
- Production of Isocitric Acid from Glucose by Candida ravautii
- A New n-Alkane Oxidation System from Pseudomonas aeruginosa S7B1
- Fermentative Production of 3-Hydroxy-3-methylglutaric Acid by Candida sorbosa
- Physical and Chemical Characterization of Cell-bound Lipases from Saccharomycopsis lipoly
- Production of Anthocyanins by Vitis Cells in Suspension Culture
- Formation and Identification of Anthocyanins ijn Cultured Cells of Vitis sp.
- Purification and Some Properties of Cell-bound Lipase from Saccharomycopsis lipolytica
- The Growth of Oxygen-resistant Hydrogen Bacterium N34 in a Jar Fermentor under High Oxygen Tension and Stability of the Oxyhydrogen Reaction
- 2-Methylcitrate Dehydratase, a New Enzyme Functioning at the Methylcitric Acid Cycle of Propionate Metabolism
- Effect of Organic Compoundson the Growth of an Obligately Autotrophic Hydrogen-Oxidizing Bacterium, Hydrogenobacter thermophilus TK-6
- Nutritional Factors Causing Mycelial Development of Saccharomycopsis lipolytica
- Application of Feeder Layer Method for Improved Colony Formation of Grape Cells and Protoplasts at Low Cell Density
- Cultural Conditions for Torulopsis Candida Cell Production from Palm Oil
- Isolation and Culture Conditions of a Thermophilic Methane-oxidizing Bacterium
- The Deficient Carbohydrate Metabolic Pathways and the Incomplete Tricarboxylic Acid Cycle in an Obligately Autotrophic Hydrogen-oxidizing Bacterium
- Excretion of L-Tryptophan by Analogue-resistant Mutants of Pseudomonas hydrogenothermophila TH-1 in Autotrophic Cultures
- Screening of Yeasts and Cultural Conditions for Cell Production from Palm Oil
- 2-Methylthio-1, 4-naphthoquinone, a New Quinone from an Extremely Thermophilic Hydrogen Bacterium
- Rapid and Dense Culture of Acinetobacter calcoaceticus on Palm Oil
- Acidic Polysaccharide Production from Methane by a New Methane-oxidizing Bacterium H-2