The Effects of Culture Conditions on the Secretion of Human Lysozyme by Saccharomyces cerevisiae A2-1-1A(Biological Chemistry)
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概要
- 論文の詳細を見る
The effects of initial glucose concentrations on the cell growth, glucose usage, and human lysozyme (HLY) secretion under the ENO1 promoter were examined in the Saccharomyces cerevisiae strain A2-1-1A harboring a multicopy plasmid. By increasing the initial glucose from 2% to 10%, the HLY secretion increased 7〜8 fold although the cell growth was not affected. By adding a mixture of mineral salts to the basal medium, the HLY secretion was increased about twice due to the continuity of the HLY expression at the stationary phase of cell growth. The high HLY secretion (5.5mg per liter, 47-fold higher than the original level) was achieved by the strain A2-1-1A grown in the synthetic basal medium containing 10% initial glucose, and supplemented with mineral salts containing ammonium sulfate, potassium phosphate, potassium chloride, magnesium sulfate, and iron sulfate.
- 社団法人日本農芸化学会の論文
- 1989-10-23
著者
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Nakahara Tadaatsu
Institute of Applied Biochemistry, University of Tsukuba
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Nakahara T
Univ. Tsukuba Ibaraki Jpn
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Nakahara Tadaatsu
Institute Of Applied Biochemistry University Of Tsukuba
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Komiya Koji
Gene Technology Section Biological Chemistry Division National Chemical Laboratory For Industry:(pre
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ICHIKAWA Kimihisa
Institute of Applied Biochemistry, University of Tsukuba
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SUZUKI Katsunori
Gene Technology Section, Biological Chemistry Division, National Chemical Laboratory for Industry
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JIGAMI Yoshifumi
Gene Technology Section, Biological Chemistry Division, National Chemical Laboratory for Industry
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Jigami Yoshifumi
Gene Technology Section Biological Chemistry Division National Chemical Laboratory For Industry
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Ichikawa K
Institute Of Applied Biochemistry University Of Tsukuba
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Ichikawa Kimihisa
Institute Of Applied Biochemistry University Of Tsukuba
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Nakahara Tadaatsu
Institute Of Applied Biochemistry The University Of Tsukuba
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Suzuki Katsunori
Gene Technology Section Biological Chemistry Division National Chemical Laboratory For Industry
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