Efficient Conversion of Itaconic Acid to (S)-(+)-Citramalic Acid by Alcaligenes xylosoxydans IL142
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概要
- 論文の詳細を見る
The effective production of (S)-(+)-citramalic acid from itaconic acid with an enantiomeric purity of more than 99.9% was successfully achieved using resting cells of a newly isolated strain, Alcaligenes xylosoxydans IL142. The highest conversion activity was obtained with an itaconic acid concentration of 65.0 g・l^<-1>. After 30 h of reaction, 68.9 g・l^<-1> of (S)-(+)-citramalic acid was produced from 65.0 g・l^<-1> of itaconic acid. This is equivalent to a molar yield of 93.1%. This production process is of considerable economic significance because only very few by-products were detected. The ATP and CoA requirements for (S)-(+)-citramalic acid formation from itaconic acid were evaluated using crude cell-free enzymes. Addition of succinate enhanced the production of (S)-(+)-citramalic acid in the presence of CoA and ATP. These results suggest the existance of strong citramalyl-CoA transferase activity in the cell.
- 社団法人日本生物工学会の論文
- 2000-04-25
著者
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Ozawa T
Univ. Tsukuba Ibaraki Jpn
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Ozawa Tetsuo
Institute Of Applied Biochemistry University Of Tsukuba
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Nakajima-kambe Toshiaki
Institute Of Applied Biochemistry University Of Tsukuba
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Nakahara Tadaatsu
Institute of Applied Biochemistry, University of Tsukuba
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HE BING-FANG
Institute of Applied Biochemistry, University of Tsukuba
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Nakahara Tadaatsu
Institute Of Applied Biochemistry University Of Tsukuba
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He Bing-fang
Institute Of Applied Biochemistry University Of Tsukuba
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Nakahara Tadaatsu
Institute Of Applied Biochemistry The University Of Tsukuba
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