Enzymatic Deodorization with Variegatic Acid from Boletus subvelutipes and Its Mechanism

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概要

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• Variegatic acid (3,3,4,4-tetrahydroxypulvinic acid, VA) which causes a blueing phenomenon in mushrooms was isolated from Boletus subvelutipes. B. subvelutipes contains 916 mg VA/100 g dry weight of the fruit body. A mixture of VA and acetone powder from B. subvelutipes caused removal of methyl mercaptan (MeSH) after blueing. Optimum pH for the blueing activity by polyphenol oxidase from B. subvelutipes was 5.0 and a high deodorizing activity was observed around the optimum pH. The rate of MeSH removal with VA in 0.4 M potassium phosphate buffer (pH 8.0) was about 36 times as high as that with (−)-epigallocatechin gallate, which has been reported to have the highest deodorizing activity among tea catechins. We isolated several kinds of conjugates of VA molecules with 1-3 MeSH molecules. These results suggest that VA has a high deodorizing activity because it can easily be oxidized and can bind to MeSHs at 6 positions in the 2 dihydroxybenzene rings of VA.

• 社団法人　日本食品科学工学会の論文
• 2000-08-01

著者

• Ozawa Tetsuo

  Institute Of Applied Biochemistry University Of Tsukuba

• Negishi Osamu

  Institute Of Applied Biochemistry University Of Tsukuba

• NEGISHI Yukiko

  Institute of Nutrition Sciences, Kagawa Nutrition University

• Negishi Yukiko

  Institute Of Nutrition Sciences Kagawa Nutrition University

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