Production of 5'-Phosphodiesterase by Catharanthus roseus Cells Promoted by Heat-Degraded Products Generated from Uronic Acid

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概要

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• Polyalginate was autoclaved at 121℃ for 20 min and its molecular weight distribution was analyzed. The autoclaved alginate yielded alginate polymer, oligomer and heat degraded products (HDPs). Each of the separated substances promoted 5'-phosphodiesterase (5'-PDase) production in suspension culture of Catharanthus roseus cells. HDPs could also be generated from other uronic acids (galacturonic acid and glucuronic acid) by autoclave treatment. The most effective substance in the HDPs was isolated and characterized as trans-4,5-dihydroxy-2-cyclopenten-1-one (DHCP). The optimal conditions for DHCP production were also established (autoclaving 1 mg/ml monogalacturonic acid [pH 2] at 121℃ for 2 h). A combination of oligo-alginate (below 4 kDa) and HDPs significantly promoted the production of 5'-PDase in C. roseus. Based on the above results, a novel alginate complex that gave a 44-fold increase in 5'-PDase production by C. roseus was developed.

• 社団法人日本生物工学会の論文
• 2002-08-25

著者

• Ozawa Tetsuo

  Institute Of Applied Biochemistry University Of Tsukuba

• Aoyagi Hideki

  Institute Of Life Science And Bioengineering Graduate School Of Life And Environmental Science Unive

• Aoyagi Hideki

  Institute Of Applied Biochemistry University Of Tsukuba

• Tanaka Hideo

  Institute Of Life Science And Bioengineering Graduate School Of Life And Environmental Science Unive

• Akimoto-tomiyama Chiharu

  Institute Of Applied Biochemistry University Of Tsukuba:(present Address)department Of Biochemistry

• Tanaka Hideo

  Institute For Biological Resources And Functions National Institute Of Advanced Industrial Science A

• Tanaka Hideo

  Institute Of Applied Biochemistry University Of Tsukuba

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