Turbidimetric Measurement of Cell Biomass of Plant Cell Suspensions
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概要
- 論文の詳細を見る
A new method using a specialized cuvette in a spectrophotometer was developed for turbidimetric measurement of cell concentration in plant cell suspensions. The spectrophotometer was able to mix plant cell suspensions in the cuvette almost homogeneously, giving a stable optical density (O.D.). When mixing was done by agitation with a magnetic stirring bar in the cuvette, the O.D. decreased at slow agitation speeds as the cell aggregates sedimented. At high agitation speeds, however, the O.D. increased as cell aggregates were broken. At an optimal agitation speed (2,500 rpm) meither aggregate sedimentation nor mechanical damage occurred and the O.D. was consistent and reproducible. In cell suspensions of Catharanthus roseus and Oryza sativa, close relationshops were obtained between the values of O.D. and dry cell weight.
- 社団法人日本生物工学会の論文
- 1992-02-25
著者
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Aoyagi H
Institute Of Applied Biochemistry University Of Tsukuba
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Aoyagi Hideki
Institute Of Life Science And Bioengineering Graduate School Of Life And Environmental Science Unive
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Aoyagi Hideki
Institute Of Applied Biochemistry University Of Tsukuba
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Aoyagi H
Univ. Tsukuba Ibaraki Jpn
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Tanaka Hideo
Institute Of Life Science And Bioengineering Graduate School Of Life And Environmental Science Unive
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JITSUFUCHI TETSUYA
Institute of Applied Biochemistry, University of Tsukuba
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Jitsufuchi Tetsuya
Institute Of Applied Biochemistry University Of Tsukuba
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Tanaka H
Institute Of Applied Biochemistry University Of Tsukuba
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Tanaka Hideo
Institute For Biological Resources And Functions National Institute Of Advanced Industrial Science A
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