Acetylation of Loofa(Luffa cylindricd) Sponge as Immobilization Carrier for Bioprocesses Involving Cellulase(BIOCHEMICAL ENGINEERING)

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概要

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• The feasibility of using loofa sponge for immobilization of cellulase-producing microorganisms was investigated by acetylating loofa sponge. Acetylation was achieved by autoclaving process of loofa sponge immersed in acetic anhydride at various temperatures for various times. The degree of acetylation, as inferred by the weight percentage gain (WPG), was enhanced by increasing both temperature and the duration of acetylation. The acetylation of a piece of loofa sponge in an autoclave at 120℃ for 20 min resulted in a WPG of about 8%, which was sufficient to protect the loofa sponge against cellulose degradation. The acetylated loofa sponge prepared under this condition was not decomposed by commercial cellulase and its structure was maintained for more than 720 h during repeated-batch treatments with commercial cellulase. A flocculating yeast(Saccharomyces cerevisiae IR-2) and a fungus(Trichoderma reesei QM9414) were successfully immobilized in the acetylated loofa sponge. In each case, the percentage of immobilized cells was as high as that obtained using nonacetylated loofa sponge. Acetylation had no adverse effects on cell growth and immobilization of T. reesei QM9414, as well as on cell growth and ethanol production by S. cerevisiae IR-2. T. reesei QM9414 immobilized on an acetylated loofa sponge was successfully used for repeated-batch cellulase production from commercial cellulose powder. Although the acetylated loofa sponge showed a slight weight loss, it was not disintegrated by activated sludge. The results obtained in this study showed that acetylated loofa sponge is suitable as an immobilization carrier for bioprocesses involving cellulase.

• 社団法人日本生物工学会の論文
• 2007-04-25

著者

• Ogbonna James

  Department Of Microbiology University Of Nigeria:university Road Nsukka

• Aoyagi Hideki

  Institute Of Life Science And Bioengineering Graduate School Of Life And Environmental Science Unive

• Aoyagi Hideki

  Life Sciences And Bioengineering Graduate School Of Life And Environmental Sciences Univ. Of Tsukuba

• Tanaka Hideo

  Institute Of Life Science And Bioengineering Graduate School Of Life And Environmental Science Unive

• TANAKA HIDEO

  Graduate School of Life and Environmental Sciences, University of Tsukuba

• Tamaki Hideyuki

  Inst. For Biological Resources And Functions National Inst. Of Advanced Industrial Sci. And Technol.

• Aoyagi Hideki

  Graduate School Of Life And Environmental Sciences University Of Tsukuba

• Hideno Akihiro

  Graduate School of Life and Environmental Sciences, University of Tsukuba

• Hideno Akihiro

  Institute Of Life Science And Bioengineering Graduate School Of Life And Environmental Science Unive

• Tamaki Hideyuki

  Aist

• Tamaki Hideyuki

  Institute For Biological Resources And Functions National Institute Of Advanced Industrial Science A

• Tamaki Hideyuki

  Bioproduction Research Institute National Institute Of Advanced Industrial Science And Technology (a

• Tanaka Hideo

  Institute For Biological Resources And Functions National Institute Of Advanced Industrial Science A

• Tamaki Hideyuki

  Institute Of Applied Biochemistry University Of Tsukuba

• Ogbonna James

  Department Of Fermentation Technology Yamanashi University

• Tanaka Hideo

  Graduate School of Economics, Kyoto University

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