Development of a Novel System for Producing Ajmalicine and Serpentine Using Direct Culture of Leaves in Catharanthus roseus Intact Plant

概要

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Due to problems of production instability, the production of plant secondary metabolites using dedifferentiated cells (callus) is not always feasible on an industrial scale. To propose a new methodology, which does not use dedifferentiated cells, a novel system for producing useful secondary metabolites using the direct culture of intact plant leaves was developed. Catharanthus roseus was used as a model medicinal plant to produce terpenoid indole alkaloids (TIAs) by suspension culture of the leaves in the phytohormone-free MS liquid medium. Adjustment of the osmotic pressure (993 kPa at 25℃) in the medium, light irradiation (60μmol m^<-2> s^<-1>) and addition of glucose (10 g/l) were effective to promote the production of TIAs such as ajmalicine (Aj) and serpentine (Sp). On the basis of semi-quantitative RT-PCR analyses, it was revealed that the culture conditions promoted gene expression of enzymes in the TIA pathway in the cultured leaves. By feeding glucose (10 g/l) on day 10 of the culture period, Aj was produced at a concentration of about 18 mg/l and Sp was produced at a concentration about 11-fold that of the control. These results represent the first step in the development of a novel production system for plant secondary metabolites.
社団法人日本生物工学会の論文
2005-03-25