Changes in the quality of antibodies produced by Chinese hamster ovary cells during the death phase of cell culture(BIOCHEMICAL ENGINEERING)
スポンサーリンク
概要
- 論文の詳細を見る
Although overproduction of recombinant proteins by mammalian cells is well established, little attention has been paid to analysis of the quality of the products. We focused on the quality of antibodies produced during the death phase of a recombinant Chinese hamster ovary (CHO) cell line. The quality of the monoclonal antibody against HM1.24 antigen and the post-translational characteristics of the subunits during CHO cell culture in a 160-L bioreactor were investigated. The culture supernatant of a stable cell line was collected and purified by affinity chromatography and then analyzed. There were no significant changes in gel-permeation chromatography variables, carbohydrate structure, or antibody-dependent cellular cytotoxicity activity during the death phase of cell culture. However, ion-exchange chromatography analysis revealed that antibody heterogeneity changed, as indicated by a decrease in cell viability. The results presented here provide useful information that will help in determining the time to end each batch culture.
著者
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KANEKO Yoshihiro
Bio-product Technology Research Department, Drug Engineering Division, Chugai Pharmaceutical Co., Lt
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SATO Ryuji
Utsunomiya Plant Manufacturing Sect. 3, Chugai Pharmaceutical Manufacturing Co., Ltd.
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AOYAGI Hideki
Life Science and Bioengineering, Graduate School of Life and Environmental Sciences, University of T
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Sato Ryuji
Utsunomiya Plant Manufacturing Section 3 Chugai Pharmaceutical Manufacturing Co. Ltd.
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Aoyagi Hideki
Life Science And Bio Engineering Laboratory Graduate School Of Life And Environmental Sciences Unive
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Kaneko Yoshihiro
Bio-product Technology Research Department Drug Engineering Division Chugai Pharmaceutical Co. Ltd.:
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Aoyagi Hideki
Life Science And Bioengineering Graduate School Of Life And Environmental Sciences University Of Tsu
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Aoyagi Hideki
Faculty Of Life And Environmental Sciences University Of Tsukuba
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- Changes in the quality of antibodies produced by Chinese hamster ovary cells during the death phase of cell culture(BIOCHEMICAL ENGINEERING)
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- Changes in the quality of antibodies produced by Chinese hamster ovary cells during the death phase of cell culture
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