Evaluation of Chinese hamster ovary cell stability during repeated batch culture for large-scale antibody production(BIOCHEMICAL ENGINEERING)
スポンサーリンク
概要
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Pharmaceutical manufacturing plants can be operated continuously for several months. It is therefore important to use cells with long-term stability for the production of active ingredients. We investigated the reliability and long-term stability of an antibody-producing cell line. A recombinant Chinese hamster ovary (CHO) cell line was cultivated in spinner flasks and reactors, including a practical production-scale reactor (1600L), for 109 days to produce monoclonal antibodies against the HM1.24 antigen. During cultivation, the cells remained stable and there was an increase in the rate of cell proliferation, yielding viable cells at high density. A decrease in cell-specific productivity was associated with this increase in the rate of cell proliferation. The cells were genetically stable and other measures of cellular function remained consistent throughout the cultivation period.
著者
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KANEKO Yoshihiro
Bio-product Technology Research Department, Drug Engineering Division, Chugai Pharmaceutical Co., Lt
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SATO Ryuji
Utsunomiya Plant Manufacturing Sect. 3, Chugai Pharmaceutical Manufacturing Co., Ltd.
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AOYAGI Hideki
Life Science and Bioengineering, Graduate School of Life and Environmental Sciences, University of T
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Sato Ryuji
Utsunomiya Plant Manufacturing Section 3 Chugai Pharmaceutical Manufacturing Co. Ltd.
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Aoyagi Hideki
Life Science And Bio Engineering Laboratory Graduate School Of Life And Environmental Sciences Unive
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Kaneko Yoshihiro
Bio-product Technology Research Department Drug Engineering Division Chugai Pharmaceutical Co. Ltd.:
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Aoyagi Hideki
Life Science And Bioengineering Graduate School Of Life And Environmental Sciences University Of Tsu
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Aoyagi Hideki
Faculty Of Life And Environmental Sciences University Of Tsukuba
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