Development of a Novel Box-Shaped Shake Flask with Efficient Gas Exchange Capacity

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概要

• 論文の詳細を見る

• A novel box-shaped shake flask with membrane filters fitted in its upper sides was developed for efficient gas exchange. During rotation, the liquid flow inside the flask is broken up at the four angles of the flask, while mambrane filters in opposite sides serve alternately as gas inlet and outlet ports, depending on the direction in which the flask is moving. Consequently, the oxygen transfer coefficients both across the gas-liquid interface and through the membrane filters are more than ten times higher than those in an Erlenmeyer flask with a cotton plug. The volumetric oxygen transfer coefficient, volatilization rate constant, and cultivation performance of representative strains of microorganisms were used to evaluate the gas exchange capacity of the newly developed shake flask. It was confirmed that the gas exchange capacity of the flask was the same as that of a jar fermentor.

• 社団法人日本生物工学会の論文
• 1998-04-25

著者

• Tanaka Hideo

  Institute Of Life Science And Bioengineering Graduate School Of Life And Environmental Science Unive

• Kato Isao

  Institute Of Applied Biochemistry University Of Tsukuba

• Kato I

  Rakuno Gakuen Univ. Hokkaido Jpn

• Tanaka Hideo

  Institute For Biological Resources And Functions National Institute Of Advanced Industrial Science A

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