The Ecological Role of a Proteolytic Psychrophile, Flavobacterium limicola Belonging to the Cytophaga-Flavobacteria Cluster, in the Decomposition of Particulate Organic Nitrogen in Freshwater Sediment

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概要

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• Microbes of the Cytophaga-Flavobacterium (CF) group, within the phylum Bacteroidetes, have been considered contributors to the early decomposition of particulate organic matter (POM) in sediments because of their abundance in the ecosystem and their great ability to degrade macromolecules on artificial media. However, there is no report on the ability of members of this group to decompose POM in sediment. We investigated the POM-decomposition capabilities of members of the CF group, Flavobacterium limicola strains ST-82^T, ST-10 and ST-92 isolated from freshwater sediment as proteolytic psychrophiles, in sterilized sediment slurries. All strains of F. limicola grew and survived at over 10^8 cfu per ml for more than 30 days in the sediment slurries at 5℃. Protease activity levels in the slurries inoculated with the strains were 3-5 times higher than the control level for over 30 days of incubation at 5℃. Concentrations of total dissolved nitrogen (TDN) released by the hydrolysis of particulate organic nitrogen (PON), significantly increased only in the slurries inoculated with F. limicola strains. Approximately 70-80% of the TDN released was converted to NH_4^+-N in these inoculated slurries. The results clearly demonstrate that F. limicola strains ST-82^T, ST-10 and ST-92 are able to secrete an extracellular protease and hydrolyze PON to TDN and thereby mineralize TDN to NH_4^+-N in freshwater sediment. This is the first report on the decomposition and mineralization of PON by members of the CF group in benthic environments.

• 日本微生物生態学会の論文

著者

• NOMURA NOBUHIKO

  Institute of Life and Environmental Sciences, University of Tsukuba

• Nakamura Kohei

  Aist:gifu Univ.

• Nomura Nobuhiko

  Graduate School Of Life And Environmental Sciences Univ. Of Tsukuba

• Nomura Nobuhiko

  Graduate School Of Life And Environmental Sciences University Of Tsukuba

• Tanaka Hideo

  Institute Of Life Science And Bioengineering Graduate School Of Life And Environmental Science Unive

• Nakamura Kazunori

  Institute For Biological Resources And Functions National Institute Of Advanced Industrial Science A

• Kamagata Yoichi

  Institute for Biological Resources and Functions, National Institute of Advanced Industrial Scinece

• Nomura Nobuhiko

  Institute Of Life And Environmental Sciences University Of Tsukuba

• Kamagata Yoichi

  AIST

• Hanada Satoshi

  Institute For Biological Resources And Functions National Institute Of Advanced Industrial Science A

• Matsumura Masatoshi

  Nstitute Of Applied Biochemistry University Of Tsukuba

• TAMAKI HIDEYUKI

  nstitute of Applied Biochemistry, University of Tsukuba

• NOMURA NAKAO

  nstitute of Applied Biochemistry, University of Tsukuba

• Tamaki Hideyuki

  Aist

• Kamagata Yoichi

  Institute For Biological Resources And Functions National Institute Of Advanced Industrial Science A

• Nomura N

  Institute Of Applied Biochemistry University Of Tsukuba

• Kamagata Yoichi

  Bioproduction Research Institute National Institute Of Advanced Industrial Science And Technology (a

• Tamaki Hideyuki

  Institute For Biological Resources And Functions National Institute Of Advanced Industrial Science A

• Tamaki Hideyuki

  Bioproduction Research Institute National Institute Of Advanced Industrial Science And Technology (a

• Kamagata Yoichi

  Institute For Biological Resources & Functions National Institute Of Advanced Industrial Science

• Tamaki Hideyuki

  Institute Of Applied Biochemistry University Of Tsukuba

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