Analysis of Dibenzothiophene Metabolic Pathway in Mycobacterium Strain G3

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概要

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• The dibenzothiophene (DBT) metabolic pathway in Mycobacterium strain G3, which is classifled as a desulfurizing microorganism with the 4S pathway, was analyzed. 2-Hydroxybiphenyl (HBP), which is an end metabolite in the DBT desulfurization reaction, and 2-methoxybiphenyl (MBP) were found in the reaction mixture, and the methoxylation pathway from HBP to MBP was clarified. Although the substrate in the methoxylation reaction was HBP, there was no relationship between expression of the methoxylation activity and that of the desulfurization activity. Then, 4,6-dimethyl DBT, 4,6-diethyl DBT and benzo[b]naphtho[2,1-d]thiophene were metabolized to their methoxy forms via the desulfurization pathway. We established the methoxylation pathway in Mycobacterium G3.

• 社団法人日本生物工学会の論文
• 2002-05-25

著者

• NOMURA NOBUHIKO

  Institute of Life and Environmental Sciences, University of Tsukuba

• Nakajima Toshiaki

  Institute Of Applied Biochemistry University Of Tsukuba

• MARUHASHI KENJI

  Bio-Refining Process Laboratory, Japan Cooperation Center, Petroleum

• Nomura Nobuhiko

  Graduate School Of Life And Environmental Sciences Univ. Of Tsukuba

• Nomura Nobuhiko

  Graduate School Of Life And Environmental Sciences University Of Tsukuba

• Nomura Nobuhiko

  Institute Of Applied Biochemistry University Of Tsukuba

• Nakahara Tadaatsu

  Institute of Applied Biochemistry, University of Tsukuba

• Okada Hideki

  Research And Development Center Cosmo Oil Co. Ltd.

• OKADA HIDEKI

  Bio-Refining Process Laboratory, Technical Cooperation Department, Japan Cooperation Center, Petrole

• Nakajima-kambe Toshiaki

  Graduate School Of Life And Environmental Sciences Univ. Of Tsukuba

• Okada Hideki

  Shin Nihon Chemical Co. Ltd

• Nakahara T

  Institute Of Applied Biochemistry University Of Tsukuba:(present Address)ryutsu Keizai University

• Nakahara Tadaatsu

  Graduate School Of Life And Environmental Science University Of Tsukuba

• Nakahara Tadaatsu

  Institute Of Applied Biochemistry University Of Tsukuba

• Nomura Nobuhiko

  Institute Of Life And Environmental Sciences University Of Tsukuba

• Maruhashi Kenji

  Bio-refining Process Laboratory Technical Cooperation Department Japan Cooperation Center

• Nakae T

  Graduate School Of Life And Environmental Sciences Univ. Of Tsukuba

• Nomura N

  Institute Of Applied Biochemistry University Of Tsukuba

• Nakahara Tadaatsu

  Institute Of Applied Biochemistry The University Of Tsukuba

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