Isolation of a Recombinant Desulfurizing 4,6-Dipropyl Dibenzothiophene in n-Tetradecane

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概要

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• Rhodococcus erythropolis strain KA2-5-1 is unable to desulfurize 4,6-dipropyl dibenzotbiophene (DBT) in the oil phase. The dsz desulfurization gene cluster from R. erythropolis strain KA2-5-1 was transferred into 22 rhodococcal and mycobacterial strains using a transposon-trans-posase complex. The recombinant strain MR65, from Mycobacterium sp. NCIMB10403, was able to grow on a minimal medium supplemented with 1.0mM 4,6-dipropyl DBT in n-tetradecane (50%, v/v) as the sole sulfur source. Resting cells of recombinant strain MR65 could desulfurize 68 mgl^<-1> of sulfur in light gas oil(LGO) containing 126mg sulfur l^<-1>. Strain MR65 had about 1.5-times the LGO desulfurization activity of R. erythropolis strain KA2-5-1. The application of a recombinant, which is able to utilize 4,6-dipropyl DBT in the oil phase, was effective in enhancing LGO biodesulfurization.

• 社団法人日本生物工学会の論文
• 2003-04-25

著者

• MARUHASHI KENJI

  Bio-Refining Process Laboratory, Japan Cooperation Center, Petroleum

• Watanabe Katsuyuki

  Pharmacological Research Laboratories Fujisawa Pharmaceutical Co. Ltd.

• Watanabe K

  Division Of Applied Bioscience Graduate School Of Agriculture Hokkaido University

• Noda K

  Bio-refining Process Laboratory Technical Cooperation Department Japan Cooperation Center

• Maruhashi Kenji

  Bio-refining Process Laboratory Technical Cooperation Department Japan Cooperation Center

• NODA KEN-ICHI

  Bio-Refining Process Laboratory Technical Cooperation Department, Japan Cooperation Center

• WATANABE KIMIKO

  Bio-Refining Process Laboratory Technical Cooperation Department, Japan Cooperation Center

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