Effects of Exogenous Phenol-Degrading Bacteria on Performance and Ecosystem of Activated Sludge
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概要
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Three exogenous phenol-degrading bacteria, Alcaligenes sp. R5,Acromobacter sp. E1 and Alcaligenes sp. E2,were each inoculated into separate laboratory units of activated sludge fed on peptone, and then phenol loading was started. The control unit (no inculum) required 10 d for complete degradation of phenol. Inoculation with strain R5 did not shorten this start-up period, whereas inoculation with strains E1 or E2 shortened it to 2 or 3 d, respectively . Population dynamics of the three strains were analyzed by enzyme-linked immunosorbent assay (ELISA) by which a population with a density of more than 1×10^8 cells per ml (background density) could be detected. The R5 population density was never greater than the background, whereas the E1 population increased to 2×10^9 cells per ml (approximately 50% of the total cell count) during the start-up period, and fell to below the background 35 d after the inoculation. The E2 pupolation initially increased to 8×10^8 cells per ml, and subsequently survived at approximately 2×10^8 cells per ml throughout the remainder of 40-d experiment. These results suggest that the inocula, strains E1 and E2,capable of forming the predominant populations, caused successful bioaugmentation which shortened the start-up period. In pure-culture experiments performed to investigate the growth of the strains on phenol and peptone, it was found that strain E1 was superior in terms of growth on peptone at low concentrations to the other strains, and strain R5 was inferior. Such an advantage of strain E1 was not observed during growth on phenol. From these results, it is proposed that one of the important properties of microorganisms for successful bioaugmentation, i.e. shortening of the start-up period, is potential for rapid growth on coexisting nutrients, such as peptone in this study.
- 社団法人日本生物工学会の論文
- 1996-09-25
著者
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Takahashi Nobuhiro
Fundamental Research Laboratories Corporate Research And Development Laboratory Tonen Corporation
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HINO SANAE
Fundamental Research Laboratories, Corporate Research and Development Laboratory, Tonen Corporation
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WATANABE KAZUYA
Fundamental Research Laboratories, Corporate Research and Development Laboratory, Tonen Corporation
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Watanabe Katsuyuki
Pharmacological Research Laboratories Fujisawa Pharmaceutical Co. Ltd.
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Watanabe K
Division Of Applied Bioscience Graduate School Of Agriculture Hokkaido University
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Hino Sanae
Fundamental Research Laboratories Corporate Research And Development Laboratory Tonen Corporation
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Watanabe K
Marine Biotechnol. Inst. Iwate Jpn
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