Nuclectide Sequence Specificity in DNA Cleavage Caused by Dihydropyrazines, a New Type of DNA Strand-Cleaving Agent
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概要
- 論文の詳細を見る
The specificity of the nucleotide sequence of DNA strand cleavage sites produced by dihydropyrazines, a new type of DNA strand-cleaving agent, was studied. Biotin-5'-end-labeled PCR-amplified DNA restriction fragments of different defined nucleotide sequences were prepared from plasmid pBR322,and reacted with dihydropyrazines in the presence of Cu^<2+>. After being heated with aqueous piperidine, the DNA products were analyzed on polyacrylamide gels. The most preferentially cleaved sites induced by dihydropyrazines were at purine/pyrimidine-guanine (5'→3') sequences. The purine/pyrimidine-adenine, pyrimidine-pyrimidine and purine-pyrimidine (5'→3') sequences were more resistant to attack by these dihydropyrazines. The side chains of the dihydropyrazine skeleton greatly affected the DNA strand-cleaving activity, and also, to some extent, the nucleotide sequence-specificity in the DNA strand-cleavage.
- 公益社団法人日本薬学会の論文
- 2000-11-01
著者
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Watanabe K
Faculty Of Pharmaceutical Science School Of Medicine Fukuoka University
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YAMAGUCHI Tadatoshi
Faculty of Pharmaceutical Sciences, Sojo University
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KASHIGE Nobuhiro
Faculty of Pharmaceutical Sciences, Fukuoka University
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YAMAGUCHI Tadatoshi
Department of Hygiene, Miyazaki Medical College
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MIAKE Fumio
Faculty of Pharmaceutical Sciences, Fukuoka University
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WATANABE Kenji
Faculty of Pharmaceutical Sciences, Fukuoka University
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Yamaguchi Tokio
Faculty Of Pharmaceutical Sciences Sojo University
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Watanabe Katsunori
Department Of Bioresources Chemistry Faculty Of Horticulture Chiba University
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Watanabe K
Tokyo Institute Of Technology Faculty Of Bioscience And Biotechnology Department Of Biological Scien
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Yamaguchi T
Tsumura Res. Laboratories Tsumura & Co.
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Watanabe K
Department Of Agriculture Tokyo University Of Agriculture
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Miake F
Faculty Of Pharmaceutical Sciences Fukuoka University
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Miake Fumio
Faculty Of Pharmaceutical Sciences Fukuoka University
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Watanabe K
Research Institute For Food Science Kyoto University:(present Address)department Of Agricultural Che
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Watanabe K
Department Of Applied Biological Sciences Faculty Of Agriculture Saga University
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Watanabe K
Marine Biotechnol. Inst. Iwate Jpn
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Watanabe K
Department Of Chemistry And Life Science College Of Bioresource Sciences Nihon University
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Tadatoshi Yamaguchi
Faculty Of Hygiene Miyazaki Medical College
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Yamaguchi Tadatoshi
Faculty Of Pharmaceutical Sciences Sojo University
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Kashige Nobuhiro
Faculty Of Pharmaceutical Sciences Fukuoka University
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Watanabe Keiichi
The Graduate School Of Science And Technology Kobe University
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Watanabe Kenji
Faculty Of Fisheries Kagoshima University
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Miake Fumio
Faculty of Pharmaceutical Science, Fukuoka University
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Kashige Nobuhiro
Faculty of Pharmaceutical Science, Fukuoka University
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WATANABE Kenji
Faculty of Agriculture, Gifu University
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