Specificity of Nucleotide Sequence in DNA Cleavage Induced by D-Glucosamine and D-Glucosamine-6-phosphate in the Presence of Cu^<2+>(Biological Chemistry)
スポンサーリンク
概要
- 論文の詳細を見る
^<32>P-End-labeled restriction fragments derived from pBR322 and pUC9 DNAs were reacted with D-glucosamine or D-glucosamine-6-phosphate in the presence of Cu^<2+>, and, after being heated at 90℃ in aqueous piperidine, the DNA products were analyzed on polyacrylamide gels for the sequencespecificity of alkali-labile cleavaged sites. The intensity of oligonucleotide bands of cleavaged sites was directly proportional to the concentration of aminosugars, indicating that the DNA cleavage was caused by the action of aminosugars themselves. The preferred DNA cleavage sites induced by these aminosugars were identical, both at pyrimidine-purine (5'→3') sequences, especially at thymineguanine ones, and to some extent at pyrimidine-pyrimidine (5'→3') sequences. The 6-phosphate moiety of D-glucosamine did not affect the specificity of DNA cleavage.
- 社団法人日本農芸化学会の論文
- 1990-02-23
著者
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Watanabe K
Faculty Of Pharmaceutical Science School Of Medicine Fukuoka University
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KASHIGE Nobuhiro
Faculty of Pharmaceutical Sciences, Fukuoka University
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WATANABE Kenji
Faculty of Pharmaceutical Sciences, Fukuoka University
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NAKASHIMA Yukihiko
Faculty of Pharmaceutical Sciences, Fukuoka University
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KOJIMA Misao
Faculty of Pharmaceutical Sciences, Fukuoka University
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Watanabe Katsunori
Department Of Bioresources Chemistry Faculty Of Horticulture Chiba University
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Kojima Misao
Faculty Of Pharmaceutical Sciences Fukuoka University
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Kojima M
Shinshu Univ. Nagano Jpn
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Watanabe K
Research Institute For Food Science Kyoto University:(present Address)department Of Agricultural Che
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Watanabe K
Marine Biotechnol. Inst. Iwate Jpn
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Kashige Nobuhiro
Faculty Of Pharmaceutical Sciences Fukuoka University
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Nakashima Yukihiko
Faculty Of Pharmaceutical Sciences Fukuoka University
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Watanabe Kenji
Faculty Of Fisheries Kagoshima University
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Kashige Nobuhiro
Faculty of Pharmaceutical Science, Fukuoka University
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WATANABE Kenji
Faculty of Agriculture, Gifu University
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