Purification and Properties of Three β-N-Acetylglucosaminidases from Lactobacillus casei ATCC 27092

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概要

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• Three β-N-acetylglucosaminidasas, GlcNAcase A, B, and C, were purified from the culture fluid of Lactobacillus casei ATCC 27092, and the molecular weights of these enzymes were estimated to be 54, 000, 51, 000, and 44, 000, resperctively, by SDS-PAGE.The production of these GlcNAcases was accelerated by the addition of N-acetylglucosamine to the culture.These enzymes had pls of adout 5.2, an optimum pH of 5.0-5.5, and an optimum temperature of 37-40゜C.The Km values of GlcNAcase A, B, and C for p-nitrophenyl-β-N-acetylglucosamine were 0.85, 1.30, and 1.04 mM and those for p-nitrophenyl-β-N-acetylgalactosamine were 39.6, 57.7, and 60.8, mM respectively.

• 社団法人日本農芸化学会の論文
• 1998-02-23

著者

• MIAKE Fumio

  Microbiology Laboratory, Faculty of Phamaceutical Sciences, School of Medicine, Fukuoka University

• WATANABE Kenji

  Microbiology Laboratory, Faculty of Pharmaceutical Sciencesm School of Medicine, Fukuoka University

• Watanabe Katsunori

  Department Of Bioresources Chemistry Faculty Of Horticulture Chiba University

• Senba M

  Fukuoka Univ. Fukuoka Jpn

• KASHIGE Nobuhiro

  Microbiology Laboratory, Faculty of Pharmaceutical Sciences, Fukuoka University

• SENBA Mio

  Microbiology Laboratory, Faculty of Pharmaceutical Sciences, Fukuoka university

• KASHIGE Noduhiro

  Microbiology Laboratory, Faculty of Pharmaceutical Sciences, Fukuoka university

• MIYAKE Fumio

  Microbiology Laboratory, Faculty of Pharmaceutical Sciences, Fukuoka university

• Miyake Fumio

  Microbiology Laboratory Faculty Of Pharmaceutical Sciences Fukuoka University

• Kashige Noduhiro

  Microbiology Laboratory Faculty Of Pharmaceutical Sciences Fukuoka University

• Watanabe Kenji

  Microbiology Laboratory Faculty Of Pharmaceutical Sciences Fukuoka University

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