Calcium Requirement for Protoplast Transfection Mediated by Polyethylene Glycol of Lactobacillus casei by PL-1 Phage DNA

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概要

• 論文の詳細を見る

• The effects of some divalent cations on protoplast transfection mediated by polyethylene glycol of Lactobacillus casei ATCC 27092 by PL-1 phage DNA in 50 mM Tris-maleate buffer (pH 6.0) were investigated. The efficiency of transfection increased about 30 times in the presence of 10 mM Ca^<2+>. Sr^<2+> increased the transfection rate as well, but Ba^<2+>, Mn^<2+>, and Mg^<2+> did not. Co^<2+> and Zn^<2+> inhibited transfection. The simultaneous use of Ca^<2+> and Mg^<2+> increased the transfection efficiency. Impairment of transfection caused by lack of Ca^<2+> could not be reversed by the addition of Ca^<2+> later. A decrease in the Ca^<2+> concentration to an ineffective level before transfection ended immediately inhibited transfection. Protoplasts were transfected with a phage adsorption mutant resistant to PL-1,also, and these metal ions had the same effect. Multiplication of phages in the transfected protoplasts was independent of the presence or absence of calcium ions. Calcium ions seemed to be involved in the entry of PL-1 DNA into the host protoplasts.

• 社団法人日本農芸化学会の論文
• 1992-11-23

著者

• Watanabe K

  Faculty Of Pharmaceutical Science School Of Medicine Fukuoka University

• MIAKE Fumio

  Faculty of Pharmaceutical Sciences, Fukuoka University

• WATANABE Kenji

  Faculty of Pharmaceutical Sciences, Fukuoka University

• KAKITA YUKARI

  Faculty of Pharmaceutical Science, School of Medicine, Fukuoka University

• NAKASHIMA Yukihiko

  Faculty of Pharmaceutical Sciences, Fukuoka University

• Watanabe Katsunori

  Department Of Bioresources Chemistry Faculty Of Horticulture Chiba University

• Miake F

  Faculty Of Pharmaceutical Sciences Fukuoka University

• Miake Fumio

  Faculty Of Pharmaceutical Sciences Fukuoka University

• Kakita Yukari

  Faculty Of Pharmaceutical Science School Of Medicine Fukuoka University

• Watanabe K

  Research Institute For Food Science Kyoto University:(present Address)department Of Agricultural Che

• Watanabe K

  Marine Biotechnol. Inst. Iwate Jpn

• Nakashima Yukihiko

  Faculty Of Pharmaceutical Sciences Fukuoka University

• Watanabe Kenji

  Faculty Of Fisheries Kagoshima University

• Miake Fumio

  Faculty of Pharmaceutical Science, Fukuoka University

• WATANABE Kenji

  Faculty of Agriculture, Gifu University

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