Expression and Secretion of Cholera Toxin B Subunit in Lactobacilli
スポンサーリンク
概要
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Lactic acid bacteria (LAB) are used in various fields, including in food and medical supplies. There has been a great deal of research into vaccine development using LAB as carriers due to their "generally recognized as safe" status. Cholera is an infectious disease that causes diarrhea due to cholera toxin (CT) produced by Vibrio cholerae. The pentameric cholera toxin B (CTB) subunit has no toxicity, and is used as an antigen in cholera vaccines and as a delivery molecule in vaccines to various diseases. In this study, we generated recombinant LAB expressing and secreting CTB. Here, we first report that CTB expressed and secreted from LAB bound to GM1 ganglioside. The secreted CTB was purified, and its immunogenicity was determined by intranasal administration into mice. The results of the present study suggested that it may be useful as the basis of a new oral cholera vaccine combining LAB and CTB.
著者
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MIAKE Fumio
Faculty of Pharmaceutical Sciences, Fukuoka University
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Arakawa Takeshi
Molecular Microbiology Group Department Of Tropical Infectious Diseases Center Of Molecular Bioscien
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Toda Akihisa
Department Of Hygienic Chemistry Daiichi University Of Pharmaceutical Sciences
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Nakashima Yukihiko
Faculty Of Pharmaceutical Sciences Fukuoka University
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Miake Fumio
Faculty of Pharmaceutical Science, Fukuoka University
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Kashige Nobuhiro
Faculty of Pharmaceutical Science, Fukuoka University
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Fukumitsu Yuki
Faculty of Pharmaceutical Sciences, Fukuoka University
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Miyata Takeshi
Molecular Microbiology Group, Department of Tropical Infectious Diseases, COMB, Tropical Biosphere Research Center, University of the Ryukyus
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Okuno Takahiro
Faculty of Pharmaceutical Sciences, Fukuoka University
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Satho Tomomitsu
Faculty of Pharmaceutical Sciences, Fukuoka University
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Irie Keiichi
Faculty of Pharmaceutical Sciences, Fukuoka University
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Hiramatsu Yukihiro
Faculty of Pharmaceutical Sciences, Fukuoka University
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Sharmin Tanjina
Faculty of Pharmaceutical Sciences, Fukuoka University
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Uyeda Saori
Faculty of Pharmaceutical Sciences, Fukuoka University
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Yamada Seitaro
Molecular Microbiology Group, Department of Tropical Infectious Diseases, COMB, Tropical Biosphere Research Center, University of the Ryukyus
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Harakuni Tetsuya
Molecular Microbiology Group, Department of Tropical Infectious Diseases, COMB, Tropical Biosphere Research Center, University of the Ryukyus
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Imoto Masumi
Department of Hygienic Chemistry, Daiichi University of Pharmacy
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Arakawa Takeshi
Molecular Microbiology Group, Department of Tropical Infectious Diseases, COMB, Tropical Biosphere Research Center, University of the Ryukyus
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