Protoplast Transfection of Lactobacillus casei by Phage PL-1 DNA(Microbiology & Fermentation Industry)
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概要
- 論文の詳細を見る
Polyethylene glycol (PEG) mediated transfection of Lactobacillus casei ATCC 27092 protoplasts by phage PL-1 DNA was done. The protoplasts were obtained by treatment with purified PL-1 phage N-acetylmuramidase in the presence of citrate. Optimum conditions for transfection were 50 % PEG 4,000, 15 μg protamine sulfate/ml, 0.15 M sucrose, and 10mM MgSO_4 in MR medium (pH 6.0). The extent of transfection was proportional to the amounts of DNA added, and the greatest efficiency of transfection after a 10-min incubation was about 3.3×10^5 PFU/μg DNA. The eclipse period of growth of progeny phases in the transfectants was 3 hr and the average burst size was 200.
- 社団法人日本農芸化学会の論文
- 1990-04-23
著者
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Watanabe K
Faculty Of Pharmaceutical Science School Of Medicine Fukuoka University
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WATANABE Kenji
Faculty of Pharmaceutical Sciences, Fukuoka University
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KAKITA YUKARI
Faculty of Pharmaceutical Science, School of Medicine, Fukuoka University
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NAKASHIMA Yukihiko
Faculty of Pharmaceutical Sciences, Fukuoka University
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SASAKI Takashi
Meiji Institute of Health Science
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Watanabe Katsunori
Department Of Bioresources Chemistry Faculty Of Horticulture Chiba University
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Kakita Yukari
Faculty Of Pharmaceutical Science School Of Medicine Fukuoka University
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Watanabe K
Research Institute For Food Science Kyoto University:(present Address)department Of Agricultural Che
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Watanabe K
Marine Biotechnol. Inst. Iwate Jpn
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Nakashima Yukihiko
Faculty Of Pharmaceutical Sciences Fukuoka University
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Watanabe Kenji
Faculty Of Fisheries Kagoshima University
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WATANABE Kenji
Faculty of Agriculture, Gifu University
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