Characteristics of Psychrophilic Alkaline Phosphatase

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概要

• 論文の詳細を見る

• The phosphatase of a psychrophile (Shewanella sp.) was purified by ammonium sulfate fractionation, followed by sequential column chromatographies. The purified enzyme was electrophoretically homogeneous on native- and SDS-PAGE. Its molecular weight was 41,826 by its amino acid composition. The enzyme had its optimal pH for the activity at 9.8,and a broad substrate specificity to dephosphorylate ATP, pyrophosphate, glycerophosphate, and so on. Its activity was increased by metal ions including Mg<2+>, Mn<2+>, and Co<2+>. The maximal activity was observed at 40℃, and the enzyme at 0℃ showed 39% of activity at 40℃. The enzyme, however, tended to lose its activity at 20℃ and pH 9.8. These results indicated that purified enzyme was an alkaline phosphatase with characteristics ; high catalytic efficiency at low temperature and gradual inactivation at an intermediate temperature.

• 社団法人日本農芸化学会の論文
• 1998-11-23

著者

• Ishida Yasuo

  Agricultural Research Laboratories Agro Division Takeda Chemical Industries Ltd.

• Watanabe K

  Faculty Of Pharmaceutical Science School Of Medicine Fukuoka University

• Ishida Yoshihiro

  Laboratory Of Biochemistry Department Of Biofunctional Chemistry Faculty Of Agriculture Kobe Univers

• TSURUTA Hiroki

  Laboratory of Biological Chemistry, Department of Biofunctional Chemistry, Faculty of Agriculture, K

• TSUNETA Sofia

  Laboratory of Biological Chemistry, Department of Biofunctional Chemistry, Faculty of Agriculture, K

• UNO Tomohide

  Department of Applied Bioscience, Faculty of Agriculture, Saga University

• WATANABE Keiichi

  Laboratory of Biological Chemistry, Department of Biofunctional Chemistry, Faculty of Agriculture, K

• AIZONO Yasuo

  Laboratory of Biological Chemistry, Department of Biofunctional Chemistry, Faculty of Agriculture, K

• Watanabe Katsunori

  Department Of Bioresources Chemistry Faculty Of Horticulture Chiba University

• Uno Tomohide

  Laboratory Of Biochemistry Department Of Biofunctional Chemistry Faculty Of Agriculture Kobe Univers

• Aizono Y

  Laboratory Of Biological Chemistry Department Of Biofunctional Chemistry Faculty Of Agriculture Kobe

• Aizono Yasuo

  Lab. Of Biological Chemistry Dep. Of Biofunctional Chemistry Graduate School Of Agricultural Sci. Ko

• Aizono Yasuo

  Laboratory Of Biological Chemistry Department Of Biofunctinal Chemistry Faculty Of Agriculture Kobe

• Tsuneta Sofia

  Laboratory Of Biochemistry Department Of Biofunctional Chemistry Faculty Of Agriculture Kobe Univers

• Tsuruta Hiroki

  Center For Cooperative Research And Development Kobe University

• Tsuruta Hiroki

  Laboratory Of Biological Chemistry Department Of Biofunctional Chemistry Faculty Of Agriculture Kobe

• Watanabe K

  Research Institute For Food Science Kyoto University:(present Address)department Of Agricultural Che

• Watanabe K

  Department Of Applied Biological Sciences Faculty Of Agriculture Saga University

• Watanabe K

  Marine Biotechnol. Inst. Iwate Jpn

• Watanabe K

  Department Of Chemistry And Life Science College Of Bioresource Sciences Nihon University

• Watanabe Keiichi

  Laboratory Of Biochemistry Faculty Of Agriculture Kyushu University

• Watanabe Keiichi

  The Graduate School Of Science And Technology Kobe University

• Tsuruta Hiroki

  Laboratory of Biochemistry, Department of Biofunctional Chemistry, Faculty of Agriculture, Kobe University

• Aizono Yasuo

  Laboratory of Biochemistry, Department of Biofunctional Chemistry, Faculty of Agriculture, Kobe University

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