Isolation and Partial Characterization of Three Protein-synthesis Inhibitory Proteins from the Seeds of Luffa cylindrica(Biological Chemistry)

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概要

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• Three new proteins which inhibit protein synthesis in rabbit reticulocyte lysates were isolated from an extract of sponge gourd (Luffa cylindricd) seeds by chromatography on a AF-Blue Toyopearl column followed by FPLC with a Mono S column. These three protein-synthesis inhibitory proteins (PSIs) have molecular masses of 19 kDa, 15 kDa, and 9 kDa, and were designated 19K-PSI, 15K-PSI, and 9K-PSI, respectively. Although the 19K-PSI had no effect on protein synthesis in HeLa cells, its inhibitory activity on the cell-free protein synthesis was 340- and 83-fold stronger than those of ricin A-chain and luffin-a, respectively, probably due to hydrolyzing mRNA. The inhibitory activities of 15K-and 9K-PSIs on the cell-free protein synthesis were weaker than those of ricin A-chain and luffin-a. The 19K-PSI was a glycoprotein having an ordinary amino acid composition, three intramolecular disulfide bonds and a blocked N-terminal residue, while the 15K-PSI was extraordinarily rich in glycine and the 9K-PSI in arginine and glutamic acid (and/or glutamine). The amino acid composition of 19K-PSI was: Ser_<27>Glx_3Gly_<164>Tyr_7Lys_9His_6, and that of 9K-PSI was: Asx_3Glx_<25>Pro_2Gly_5Lys_2His_2Arg_<25>Trp_3.

• 社団法人日本農芸化学会の論文
• 1990-08-23

著者

• WATANABE Keiichi

  Laboratory of Biological Chemistry, Department of Biofunctional Chemistry, Faculty of Agriculture, K

• Funatsu Gunki

  Laboratory Of Biochemistry And ** Laboratory Of Protein Chemistry & Engineering Faculty Of Agric

• Minami Yuji

  Laboratory Of Biochemistry Faculty Of Agriculture Kyushu University

• Watanabe Keiichi

  Laboratory Of Biochemistry Faculty Of Agriculture Kyushu University:(present Office)department Of Ap

• Watanabe Keiichi

  Laboratory Of Biochemistry Faculty Of Agriculture Kyushu University

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