Complete Amino Acid Sequence of Chitinase-A from Leaves of Pokeweed (Phytolacca americana)

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概要

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• The complete amino acid sequence of pokeweed leaf chitinase-A was determined. First all 11 tryptic peptides from the reduced and S-carboxymethylated form of the enzyme were sequenced. Then the same form of the enzyme was cleaved with cyanogen bromide, giving three fragments. The fragments were digested with chymotrypsin or Staphylococcus aureus V8 protease. Last, the 11 tryptic peptides were put in order. Of seven cysteine residues, six were linked by disulfide bonds (between Cys25 and Cys74,Cys89 and Cys98,and Cys195 and Cys208); Cys176 was free. The enzyme consisted of 208 amino acid residues and had a molecular weight of 22,391. It consisted of only one polypeptide chain without a chitin-binding domain. The length of the chain was almost the same as that of the catalytic domains of class IL chitinases. These findings suggested that this enzyme is a new kind of class IIL chitinase, althouth its sequence resembles that of catalytic domains of class IL chitinases more than of the class IIl chitinases reported so far. Discussion on the involvement of specific tryptophan residue in the active site of PLC-A is also given based on the sequence similarity with rye seed chitinase-c.

• 社団法人日本農芸化学会の論文
• 1998-04-23

著者

• Yanagita T

  Japan Institute Of Oil & Fats Other Foods Inspection Foundation

• FUNATSU Gunki

  Laboratory of Biochemistry, Faculty of Agriculture, Kyushu University

• ISHIGURO Masatsune

  Laboratory of Protein Chemistry and Engineering, Kyushu University Graduate School of Bioresource an

• Yanagita Teruyoshi

  Japan Institute Of Oil & Fats Other Foods Inspection Foundation

• Funatsu G

  Kyushu Univ. Fukuoka Jpn

• Funatsu Gunki

  Laboratory Of Biochemistry And ** Laboratory Of Protein Chemistry & Engineering Faculty Of Agric

• YAMAGAMI Takeshi

  Laboratories of Protein Chemistry and Engineering

• Ishiguro M

  Laboratory Of Insect Genetic Resources Faculty Of Agriculture Kyushu University

• Ishiguro M

  Kyushu Univ. Fukuoka Jpn

• Ishiguro Masatsune

  Laboratory Of Protein Chemistry & Engineering Department Of Genetic Resources Technology Facultr

• Yamagami T

  Laboratory Of Protein Chemistry And Engineering Graduate School Of Bioresource And Environmental Sci

• TANIGAWA Miho

  Laboratory of Protein Chemistry & Engineering, Department of Genetic Resources Technology, Graduate

• Tanigawa Miho

  Laboratory Of Protein Chemistry & Engineering Department Of Genetic Resources Technology Graduat

• Ishiguro Masatsune

  Laboratory of Insect Genetic Resources, Faculty of Agriculture, Kyushu University

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