Isolation and Characterization of Chitinase Isoforms from the Bulbs of Four Species of the Genus Tulipa

スポンサーリンク

概要

• 論文の詳細を見る

• Six chitinase isoforms, designated TBC-1 to TBC-6, were purified to homogenity from the bulbs of four species (Tulipa bakeri, T. tarda, T. turkestanica, and T. praestans) of the genus Tulipa by CM-cellulose column chromatography, Butyl-Toyopearl 650M hydrophobic column chromatography, gel filtration on Sephadex G-75, and Mono-S fast protein liquid chromatography (FPLC). The chitinases had molecular weights of 30, 000 and isoelectric points of 5.2 to 6.1. These chitinases were found to proteins with similar amino acid compositions and N-terminal sequences. The tulip chitinases all had two half-cystine residues, one more than gladiolus bulb class IIIb chitinase, but many fewer than chitinases of plant class I (15-17 Cys residues/mol), II (5-8 Cys residues/mol), or III (6 Cys residues/mol). The N-terminal sequences of tulip chitinases were similar to the sequence of the gladiolus chitinase, but did not resemble sequence of any class of plant chitinase. The optimal pH of these chitinases toward glycolchitin was pH 5. TBC-1 hydrolyzed (GlcNAc)_6 into (GlcNAc)_2, (GlcNAc)_3, and (GlcNAc)_4, and hydrolyzed (GlcNAc)_5 into (GlcNAc)_2 and (GlcNAc)_3.

• 社団法人日本農芸化学会の論文
• 1998-03-23

著者

• Yanagita T

  Japan Institute Of Oil & Fats Other Foods Inspection Foundation

• ASO yoichi

  Laboratory of Protein Chemistry & Engineering, Kyushu University

• ISHIGURO Masatsune

  Laboratory of Protein Chemistry and Engineering, Kyushu University Graduate School of Bioresource an

• Yanagita Teruyoshi

  Japan Institute Of Oil & Fats Other Foods Inspection Foundation

• Aso Y

  Institute Of Genetic Resources Graduate School Of Bioresources And Bioenvironmental Science Kyushu U

• YAMAGAMI Takeshi

  Laboratories of Protein Chemistry and Engineering

• Ishiguro M

  Laboratory Of Insect Genetic Resources Faculty Of Agriculture Kyushu University

• TAIRA Toki

  Department of Bioscience and Biotechnology, Faculty of Agriculture, Ryukyu University

• TAIRA Toki

  Laboratory of Protein Chemistry and Engineering, Graduate School of Bioresource and Environmental Sc

• Ishihara M

  Laboratory Of Protein Chemistry And Engineering Graduate School Of Bioresource And Environmental Sci

• Ishiguro Masatsune

  Laboratory Of Protein Chemistry & Engineering Department Of Genetic Resources Technology Facultr

• Yamagami T

  Laboratory Of Protein Chemistry And Engineering Graduate School Of Bioresource And Environmental Sci

• Taira T

  Department Of Bioscience And Biotechnology Faculty Of Agriculture Ryukyu University

• Ishiguro Masatsune

  Laboratory of Insect Genetic Resources, Faculty of Agriculture, Kyushu University

• ASO Yoichi

  Laboratory of Genetic and Protein Engineering, Faculty of Agriculture, Kyushu University

関連論文

• Study on Association Factor Using the Substituted Analogs of Tetramerization Domain of Tumor Suppressor Protein p53
• Effects of Guanidine Hydrochloride on Pyruvate Dehydrogenase Complex
• Thermally Induced Disintegration of the Bacillus stearothermophilus Dihydrolipoamide Dehydrogenase
• Guanidine Hydrochloride-Induced Changes of the E2 Inner Core of the Bacillus stearothermophilus Pyruvate Dehydrogenase Complex^1
• Potassium Iodide-Induced Changes in Pyruvate Dehydrogenase Complex from Bacillus stearothermophilus
• Further Studies on Thermal Denaturation of Pyruvate Dehydrogenase Complex from Bacillus stearothermophilus
• Homogeneity of the Pyruvate Dehydrogenase Multienzyme Complex from Bacillus stearothermophilus
• Thermal Disassembly of Pyruvate Dehydrogenase Multienzyme Complex from Bacillus stearothermophilus
• Purification of Pyruvate Dehydrogenase Complex from an Extreme Thermophile, Bacillus caldolyticus, and Its Thermal Stability
• Insulin/IGF-like Peptides of the Nematode Caenorhabditis elegans : Structures and Analyses of the Expression Pattern and Physiological Function
• Characterization of superoxide dismutase from the fall webworm, Hyphantria cunea : Comparison of its properties to superoxide dismutase from the silkworm Bombyx mori
• Expression of Prophenoloxidase mRNA during Silkworm Hemocyte Development
• Silkworm Midgut Proteins Interacting with a Hemolymph Protease Inhibitor, CI-8
• Cloning and Characterization of a Caenorhabditis elegans cDNA Encoding a New Insulin/IGF-Like Peptide(Biochemistry & Molecular Biology)
• Synthesis of Mutants of Lipophilic Core in a De Novo Designed Small Globular Protein (SGP) and Their Interactions with Lipid-and Bio-Membranes
• Mutational Analysis of Amino Acid Residues Involved in Catalytic Activity of a Family 18 Chitinase from Tulip Bulbs(Biochemistry & Molecular Biology)
• Properties of α-Mannosidase Partially Purified from the Apple Snail, Pomacea canaliculata
• Purification of Pomacea canaliculata α-Fucosidase Isoforms with Different Thermostabilities
• Structures of Insulin-like Peptides of the Nematode Caenorhabditis elegans
• Genes Encoding Small Heat Shock Proteins of the Silkworm, Bombyx mori
• Proteomic Studies of Lipopolysaccharide-induced Polypeptides in the Silkworm, Bombyx mori
• In Vivo and in Vitro Interactions of the Bombyx mori Chymotrypsin Inhibitor b1 with Escherichia coli
• Purification of Chymotrypsin Inhibitors from Larval Hemolymph of the Silkworm, Bombyx mori
• Overproduction and characterization of hydroxyphenylalanine oxidoreductase of the silkmoth
• Molecular Cloning, Functional Expression, and Mutagenesis of cDNA Encoding Class I Chitinase from Rye (Secale cereale) Seeds
• Antifungal Activity of Rye (Secale cereale) Seed Chitinases : the Different Binding Manner of Class I and Class II Chitinases to the Fungal Cell Walls(Biochemistry & Molecular Biology)
• Molecular Cloning, Functional Expression, and Mutagenesis of cDNA Encoding Rye (Secale cereale) Seed Chitinase-c(Biochemistry & Molecular Biology)
• Localization, Accumulation, and Antifungal Activity of Chitinases in Rye (Secale cereale) Seed(Biochemistry & Molecular Biology)
• Isolation and Characterization of Chitinase Isoforms from the Bulbs of Four Species of the Genus Tulipa
• Complete Amino Acid Sequence of Chitinase-a from Bulbs of Gladiolus (Gladiolus gandavensis)
• Purification and Characterization of Two Chitinase Isoforms from the Bulbs of Gladiolus (Gladiolus gandavensis)
• The Amino Acid Sequence of Mitogenic Lectin-B from the Roots of Pokeweed : Phytolacca americana
• Trans Fatty Acids in Margarines Marketed in Eleven Countries
• Behavior of Tocopherol and Vitamin K_1 in the Hydrogenation of Edible Oil
• Study of trans Fatty Acid Content in Commercial Foods in Japan: 4.Chocolate and Chocolate Confectioery 〔和文〕
• Superoxide Dismutase from the Silkworm, Bombyx mori : Sequence, Distribution, and Overexpression
• Utility of Dry Gel from Two-dimensional Electrophoresis for Peptide Mass Fingerprinting Analysis of Silkworm Proteins
• Integration of the twenty-fourth and twenty-seventh linkage groups of the silkwom, Bombyx mori
• Proteomic Studies of Isoforms of the P25 Component of Bombyx mori Fibroin
• Synthesis and Antifungal Activity of Cinnamic Acid Esters
• Kinetic Analysis of Lysozyme-Inhibitor Complex Formation
• On the Activation Process of Pepsinogen
• On the Mechanism of Lysozyme Catalysis II. Hydrolysis of Sugar Oxazoline
• Purification and Characterization of Extracellular Cysteine Protease Inhibitor, ECPI-2, from Chlorella sp.(MICROBIAL PHYSIOLOGY AND BIOTECHNOLOGY)
• Tissue Distribution, Synthesis Stage, and Ethylene Induction of Pineapple (Ananas comosus) Chitinases
• Characterization and Antifungal Activity of Gazyumaru (Ficus microcarpa) Latex Chitinases : Both the Chitin-Binding and the Antifungal Activities of Class I Chitinase Are Reinforced with Increasing Ionic Strength
• Purification, Characterization, and Antifungal Activity of Chitinases from Pineapple (Ananas comosus) Leaf
• Purification and Characterization of Extracellular Cysteine Protease Inhibitor from Chlorella sp
• Structural Properties of Silkworm Small Heat-Shock Proteins : sHSP19.9 and sHSP20.8
• Mitogenic Properties of Pokeweed Lectin-D Isoforms on Human Peripheral Blood Lymphocytes : Non-mitogen PL-D1 and Mitogen PL-D2
• Cloning, Sequencing, and Overexpression of Gene Encoding the Bacillus stearothermophilus Lipoate Acetyltransferase
• Application of the Fowler-Warten Method to a Stiff Enzymatic Reaction
• Enzymatic System Realizing Two-factor Model
• Dynamic Characteristics of Enzymatic Feedback Systems
• Threshold of Enzymatic Reaction System, Further Studies with a Simplified Model
• Response of Enzymatic Feedback System to Oscillatory Input
• Oscillatory Behavior of Enzymatic Feedback System
• Studies on the Threshold Mechanism of Enzymatic Reaction
• Expression and Characterization of a Sigma-Class Glutathione S-Transferase of the Fall Webworm, Hyphantria cunea
• Partial Purification and Characterization of DOPA Quinone Imine Conversion Factor from Larval Hemolymph of the Silkworm, Bombyx mori
• Modification of Tryptophan Residues of Plant Class III Chitinases Involved in Enzyme Activity
• Positions of Disulfide Bonds in Rye (Secale cereale) Seed Chitinase-a
• Complete Amino Acid Sequence of Chitinase-A from Leaves of Pokeweed (Phytolacca americana)
• The Complete Amino Acid Sequence of Chitinase-B from the Leaves of Pokeweed (Phytolacca americana)
• Thermally Induced Changes of Lipoate Acetyltransferase Inner Core Isolated from the Bacillus stearothermophilus Pyruvate Dehydrogenase Complex
• Purification and Characterization of Two Chitinases from the Leaves of Pokeweed (Phytolacca americana)
• Computer Analysis of Lysozyme-catalyzed Reaction with Chitooligosaccharide
• Note on Steady-state Approximation of Enzyme Kinetics
• Simulation of Biochemical System with Computer Graphics
• Involvements of Trp23 in the Chitin-binding and of Trp131 in the Chitinase Activity of Rye Seed Chitinase-a
• Identification of the Tryptophan Residue Located at the Substrate-binding Site of Rye Seed Chitinase-c
• Purification and Characterization of Intracellular Cysteine Protease Inhibitor from Chlorella sp
• Purification and Characterization of Feruloyl Esterase from Aspergillus awamori
• Limited Proteolysis and Reduction-carboxymethylation of Rye Seed Chitinase-a: Role of the Chitin-binding Domain in Its Chitinase Action
• The Complete Amino Acid Sequence of Chitinase-a from the Seeds of Rye (Secale cereal)
• The Complete Amino Acid Sequence of Chitinase-c from the Seeds of Rye (Secale cereal)
• Identification of the Aspartic Acid Residue Located at or near Substrate-binding Site of Rye Seed Chitinase-c
• Cloning, Sequencing, and Expression of the Tulip Bulb Chitinase-1 cDNA
• Purification and Some Properties of Three Chitinases from the Seeds of Rye (Secale cereale)
• Complete Amino Acid Sequences of Chitinase-1 and -2 from Bulbs of Genus Tulipa
• Disintegration of Uncooked Rice by Carboxymethyl Cellulase from Sporotrichum sp.HG-I
• Synthesis and Fungicidal Activity of 6-Alkyl Six-membered Cyclic Thiophosphates
• Purification and Some Properties of a Thermostable Xylanase from thermophilic Fungus Strain HG-1
• Syntheses and Biological Activities of Dihydro-5,6-dehydrokawain Derivatives
• A Ricin B chain-Ribonuclease A Hybrid Protein : A new tool for introduction of a foreign protein into targeted cells
• Base-catalyzed Intramolecular Rearrangement of Trimethylamine N-oxide

もっと見る 閉じる

スポンサーリンク