Purification and Characterization of Extracellular Cysteine Protease Inhibitor, ECPI-2, from Chlorella sp.(MICROBIAL PHYSIOLOGY AND BIOTECHNOLOGY)
スポンサーリンク
概要
- 論文の詳細を見る
An extracellular cysteine protease inhibitor (ECPI-2) was purified to homogeneity from the culture filtrate of Chlorella sp. 4533 by the combination of various column chromatographies. The molecular mass of the inhibitor was estimated to be 340 kDa by SDS-PAGE. The inhibitor was extremely heat-stable under acidic or neutral condition. ECPI-2 exhibited an inhibitory activity against the proteolytic activity of papain, ficin, or chymopapain, but not against stem bromelain or cathepsin B. The inhibitor showed no inhibitory activity against trypsin, α-chymotrypsin or thermolysin. ECPI-2 contains 33.6% carbohydrate residues by weight and inhibits papain at a molar ratio of 1 : 2. The proteolysis of the inhibitor by trypsin or α-chymotrypsin was apparent, but the inhibitory activity of ECPI-2 was unaffected by these enzymes. The α-chymotrypsin hydrolysis product from ECPI-2 was further separated into six fractions by gel filtration. From these results, it is suggested that ECPI-2 has several reactive sites for papain.
- 社団法人日本生物工学会の論文
- 2006-02-25
著者
-
TAWATA Shinkichi
Department of Bioscience and Biotechnology, Faculty of Agriculture, University of the Ryukyus
-
TAIRA Toki
Department of Bioscience and Biotechnology, Faculty of Agriculture, Ryukyu University
-
ISHIHARA Masanobu
Department of Bioscience and Biotechnology, Faculty of Agriculture, Ryukyu University
-
Ishihara Masanobu
Department Of Bioscience And Biotechnology Faculty Of Agriculture Ryukyu University
-
Shiroma Tomoaki
Department of Bioscience and Biotechnology, Faculty of Agriculture, University of the Ryukyus
-
Shiroma Tomoaki
Department Of Bioscience And Biotechnology Faculty Of Agriculture University Of The Ryukyus
-
Taira Toki
Department Of Bioscience And Biotechnology Faculty Of Agriculture Ryukyu University
-
Taira Toki
Department Of Bioscience And Biotechnology Faculty Of Agriculture University Of The Ryukyus
-
Tawata Shinkichi
Department Of Bioscience And Biotechnology College Of Agriculture University Of Ryukyus
関連論文
- Efficacy of extracting solvents to chemical components of kava (Piper methysticum) roots
- Current status of biological control of paddy weeds in Vietnam
- Antioxidant and Antibacterial Activities of Rumex japonicus HOUTT. Aerial Parts(Pharmacology)
- Antibacterial Activity of Flavonoids against Staphylococcus epidermidis, a Skin Bacterium(Organic Chemistry)
- Molecular Cloning, Functional Expression, and Mutagenesis of cDNA Encoding Class I Chitinase from Rye (Secale cereale) Seeds
- Antifungal Activity of Rye (Secale cereale) Seed Chitinases : the Different Binding Manner of Class I and Class II Chitinases to the Fungal Cell Walls(Biochemistry & Molecular Biology)
- Molecular Cloning, Functional Expression, and Mutagenesis of cDNA Encoding Rye (Secale cereale) Seed Chitinase-c(Biochemistry & Molecular Biology)
- Localization, Accumulation, and Antifungal Activity of Chitinases in Rye (Secale cereale) Seed(Biochemistry & Molecular Biology)
- Isolation and Characterization of Chitinase Isoforms from the Bulbs of Four Species of the Genus Tulipa
- Synthesis and Antifungal Activity of Cinnamic Acid Esters
- Purification and Characterization of Extracellular Cysteine Protease Inhibitor, ECPI-2, from Chlorella sp.(MICROBIAL PHYSIOLOGY AND BIOTECHNOLOGY)
- Tissue Distribution, Synthesis Stage, and Ethylene Induction of Pineapple (Ananas comosus) Chitinases
- Characterization and Antifungal Activity of Gazyumaru (Ficus microcarpa) Latex Chitinases : Both the Chitin-Binding and the Antifungal Activities of Class I Chitinase Are Reinforced with Increasing Ionic Strength
- Purification, Characterization, and Antifungal Activity of Chitinases from Pineapple (Ananas comosus) Leaf
- Purification and Characterization of Extracellular Cysteine Protease Inhibitor from Chlorella sp
- Purification and Properties of a Ribonuclease from a Species of the Genus Monascus
- Pc5 EFFECT OF PLANT FLAVONOIDS ON DNA AND RNA POLYMERASES
- Allelochemicals of barnyardgrass-infested soil and their activities on crops and weeds
- Inhibitory Effects of Alpinia speciosa K. SCHUM on the Porphyrin Photooxidative Reaction
- Angiotensin I-Converting Enzyme Inhibitory Peptides Isolated from Tofuyo Fermented Soybean Food(Food & Nutrition Science)
- Purification and Characterization of Intracellular Cysteine Protease Inhibitor from Chlorella sp
- Purification and Characterization of Feruloyl Esterase from Aspergillus awamori
- Herbicidal and fungicidal activities and identification of potential phytotoxins from Bidens pilosa L. var. radiata Scherff
- Disintegration of Uncooked Rice by Carboxymethyl Cellulase from Sporotrichum sp.HG-I
- Synthesis and Fungicidal Activity of 6-Alkyl Six-membered Cyclic Thiophosphates
- Purification and Some Properties of a Thermostable Xylanase from thermophilic Fungus Strain HG-1
- Syntheses and Biological Activities of Dihydro-5,6-dehydrokawain Derivatives
- Antifungal Activity of Recombinant Class V Chitinases from Nicotiana tabacum and Arabidopsis thaliana
- Significant Longevity-Extending Effects of Alpinia zerumbet Leaf Extract on the Life Span of Caenorhabditis elegans