Purification, Characterization, and Antifungal Activity of Chitinases from Pineapple (Ananas comosus) Leaf
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概要
- 論文の詳細を見る
Three chitinases, designated pineapple leaf chitinase (PL Chi)-A, -B, and -C were purified from the leaves of pineapple (Ananas comosus) using chitin affinity column chromatography followed by several column chromatographies. PL Chi-A is a class III chitinase having a molecular mass of 25 kDa and an isoelectric point of 4.4. PL Chi-B and -C are class I chitinases having molecular masses of 33 kDa and 39 kDa and isoelectric points of 7.9 and 4.6 respectively. PL Chi-C is a glycoprotein and the others are simple proteins. The optimum pHs of PL Chi-A, -B, and -C toward glycolchitin are pH 3, 4, and 9 respectively. The chitin-binding ability of PL Chi-C is higher than that of PL Chi-B, and PL Chi-A has lower chitin-binding ability than the others. At low ionic strength, PL Chi-B exhibits strong antifungal activity toward Trichoderma viride but the others do not. At high ionic strength, PL Chi-B and -C exhibit strong and weak antifungal activity respectively. PL Chi-A does not have antifungal activity.
- 社団法人 日本農芸化学会の論文
- 2005-01-23
著者
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TAIRA Toki
Department of Bioscience and Biotechnology, Faculty of Agriculture, Ryukyu University
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ISHIHARA Masanobu
Department of Bioscience and Biotechnology, Faculty of Agriculture, Ryukyu University
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Ishihara Masanobu
Department Of Bioscience And Biotechnology Faculty Of Agriculture Ryukyu University
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TOMA Noriko
Department of Bioscience and Biotechnology, Faculty of Agriculture, Ryukyu University
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Taira Toki
Department Of Bioscience And Biotechnology Faculty Of Agriculture Ryukyu University
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Toma Noriko
Department Of Bioscience And Biotechnology Faculty Of Agriculture Ryukyu University
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Taira T
Department Of Bioscience And Biotechnology Faculty Of Agriculture Ryukyu University
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