Purification and Properties of a Ribonuclease from a Species of the Genus Monascus

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概要

• 論文の詳細を見る

• Ribonuclease was purified to homogeneity from Monascus sp. No. 3403 by column chromatography on DEAE-cellulose, DEAE-Toyopearl 650S, 5'-AMP Sepharose 4B, and Sephadex G-75. The molecular weight of the purified enzyme was estimated to be 30,000by gel filtration and 26,000 by Sodium dodmlecyl sulfate poly-acrylamide gel electrophoresis. The enzyme contained 5.0% car-bohydrate. Homopolyadenylic acid was a good substrate for the enzyme. The optimum pH of the enzyme was 4.2 and its optimum temperature was 55℃.

• 社団法人日本農芸化学会の論文
• 1995-02-23

著者

• YASUDA Masaaki

  Department of Bioscience and Biotechnology, Faculty of Agriculture, University of the Ryukyus

• TAWATA Shinkichi

  Department of Bioscience and Biotechnology, Faculty of Agriculture, University of the Ryukyus

• Yasuda M

  Department Of Microbiology Osaka University Of Pharmaceutical Sciences

• Yasuda Masaaki

  Department Of Bioscience And Biotechnology Faculty Of Agriculture University Of The Ryukyus

• KOBAMOTO Naotada

  Department of Bioscience and Biotechnology, College of Agriculture, University of the Ryukyus

• TOYAMA Seizen

  Department of Bioscience and Biotechnology, College of Agriculture, University of the Ryukyus

• Toyama Seizen

  Department Of Bioscience And Biotechnology Faculty Of Agriculture University Of The Ryukyus

• Ikehara Kayoko

  Department of Bioscience and Biotechnology, University of the Ryukyus

• Tawata S

  Univ. Ryukyus Okinawa Jpn

• Tawata Shinkichi

  Department Of Bioscience And Biotechnology College Of Agriculture University Of Ryukyus

• Ikehara Kayoko

  Department Of Bioscience And Biotechnology University Of The Ryukyus

• Kobamoto Naotada

  Department Of Bioscience And Biotechnology Technology College Of Agriculture University Of The Ryuky

• Kobamoto Naotada

  Department Of Agricultural Chemistry College Of Agriculture University Of The Ryukyus

• Yasuda Masaaki

  Department Of Bioscience And Biotechnology Faculty Of Agriculture University Of Ryukyus

• TOYAMA Seizen

  Department of Agricultural Chemistry, University of the Ryukyus

• YASUDA Masaaki

  Department of Agricultural Chemistry, University of the Ryukyus

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