Further Studies on Thermal Denaturation of Pyruvate Dehydrogenase Complex from Bacillus stearothermophilus

スポンサーリンク

概要

• 論文の詳細を見る

• Thermally induced changes in pyruvate dehydrogenase complex (PDC) from B. stearothermophilus were examined mainly at temperatures from 60゜to 70℃. Accompanied by inactivation of pyruvate decarboxylase, light scattering decreased, and ANS fluorescence increased. These changes including the inactivation were approximately first-order reactions, and the values of rate constants were greatly dependent on termperature. Chromatographic studies showed that any polypeptides were in associated forms and that final products were aggregates (> 230S) and an assembly (48S) smaller than PDC. The aggregates and assembly were rich in decarboxylase and lipoate acetyltransferase, respectively. It was suggested that, during the thermal denaturation, a decarboxylase was dissociated from PDC and immediately involved in aggregates.

• 1997-07-23

著者

• YAMASHITA Shoji

  Institute of Biophysics, Faculty of Agriculture, Kyushu University

• ASO yoichi

  Laboratory of Protein Chemistry & Engineering, Kyushu University

• Yamashita Shoji

  Faculty Of Agriculture Graduate School Of Kyushu Univ

• ISHIGURO Masatsune

  Laboratory of Protein Chemistry and Engineering, Kyushu University Graduate School of Bioresource an

• Aso Y

  Institute Of Genetic Resources Graduate School Of Bioresources And Bioenvironmental Science Kyushu U

• Ishiguro M

  Laboratory Of Insect Genetic Resources Faculty Of Agriculture Kyushu University

• HIROMASA Yasuaki

  Laboratory of Protein Chemistry and Engineering, Graduate School of Genetic Resources Technology, Ky

• AIKAWA Yoshikatsu

  Laboratory of Protein Chemistry and Engineering, Graduate School of Genetic Resources Technology, Ky

• Yamashita Shoji

  Institute Of Biophysics Faculty Of Agriculture Graduate School Of Kyushu University

• Ishiguro Masatsune

  Laboratory Of Protein Chemistry & Engineering Department Of Genetic Resources Technology Facultr

• Ishiguro M

  Suntory Institute For Bioorganic Research

• Aikawa Yoshikatsu

  Laboratory Of Protein Chemistry And Engineering Graduate School Of Genetic Resources Technology Kyus

• Hiromasa Yasuaki

  Laboratory Of Protein Chemistry And Engineering Department Of Genetic Resources Technology Graduate

• Ishiguro Masatsune

  Laboratory of Insect Genetic Resources, Faculty of Agriculture, Kyushu University

• ASO Yoichi

  Laboratory of Genetic and Protein Engineering, Faculty of Agriculture, Kyushu University

関連論文

• 2P-036 ニガウリトリプシンインヒビターにおけるTrp-Trp間エネルギー移動(蛋白質-物性(安定性,折れたたみなど),第47回日本生物物理学会年会)
• Resolution and Characterization of Tryptophyl Fluorescence of Hen Egg-White Lysozyme by Quenching-and Time-Resolved Spectroscopy
• Study on Association Factor Using the Substituted Analogs of Tetramerization Domain of Tumor Suppressor Protein p53
• Effects of Guanidine Hydrochloride on Pyruvate Dehydrogenase Complex
• Thermally Induced Disintegration of the Bacillus stearothermophilus Dihydrolipoamide Dehydrogenase
• Guanidine Hydrochloride-Induced Changes of the E2 Inner Core of the Bacillus stearothermophilus Pyruvate Dehydrogenase Complex^1
• Potassium Iodide-Induced Changes in Pyruvate Dehydrogenase Complex from Bacillus stearothermophilus
• Further Studies on Thermal Denaturation of Pyruvate Dehydrogenase Complex from Bacillus stearothermophilus
• Homogeneity of the Pyruvate Dehydrogenase Multienzyme Complex from Bacillus stearothermophilus
• Thermal Disassembly of Pyruvate Dehydrogenase Multienzyme Complex from Bacillus stearothermophilus
• Purification of Pyruvate Dehydrogenase Complex from an Extreme Thermophile, Bacillus caldolyticus, and Its Thermal Stability
• The Partially Unfolded State of β-Momorcharin Characterized with Steady-state and Time-resolved Fluorescence Studies
• 2P009 Fluorescence spectroscopic studies on the structure-dynamics-function correlation of ribosome-inactivating proteins(29. Protein structure and dynamics (II),Poster Session,Abstract,Meeting Program of EABS & BSJ 2006)
• Insulin/IGF-like Peptides of the Nematode Caenorhabditis elegans : Structures and Analyses of the Expression Pattern and Physiological Function
• Characterization of superoxide dismutase from the fall webworm, Hyphantria cunea : Comparison of its properties to superoxide dismutase from the silkworm Bombyx mori
• Expression of Prophenoloxidase mRNA during Silkworm Hemocyte Development
• Silkworm Midgut Proteins Interacting with a Hemolymph Protease Inhibitor, CI-8
• 2P-035 GFP融合蛋白質GFP-GSTのナノ秒回転ダイナミクス(蛋白質-物性(安定性,折れたたみなど),第47回日本生物物理学会年会)
• Cloning and Characterization of a Caenorhabditis elegans cDNA Encoding a New Insulin/IGF-Like Peptide(Biochemistry & Molecular Biology)
• Synthesis of Mutants of Lipophilic Core in a De Novo Designed Small Globular Protein (SGP) and Their Interactions with Lipid-and Bio-Membranes
• Mutational Analysis of Amino Acid Residues Involved in Catalytic Activity of a Family 18 Chitinase from Tulip Bulbs(Biochemistry & Molecular Biology)
• Properties of α-Mannosidase Partially Purified from the Apple Snail, Pomacea canaliculata
• Purification of Pomacea canaliculata α-Fucosidase Isoforms with Different Thermostabilities
• Structures of Insulin-like Peptides of the Nematode Caenorhabditis elegans
• Genes Encoding Small Heat Shock Proteins of the Silkworm, Bombyx mori
• Proteomic Studies of Lipopolysaccharide-induced Polypeptides in the Silkworm, Bombyx mori
• In Vivo and in Vitro Interactions of the Bombyx mori Chymotrypsin Inhibitor b1 with Escherichia coli
• Purification of Chymotrypsin Inhibitors from Larval Hemolymph of the Silkworm, Bombyx mori
• Overproduction and characterization of hydroxyphenylalanine oxidoreductase of the silkmoth
• Molecular Cloning, Functional Expression, and Mutagenesis of cDNA Encoding Class I Chitinase from Rye (Secale cereale) Seeds
• Antifungal Activity of Rye (Secale cereale) Seed Chitinases : the Different Binding Manner of Class I and Class II Chitinases to the Fungal Cell Walls(Biochemistry & Molecular Biology)
• Molecular Cloning, Functional Expression, and Mutagenesis of cDNA Encoding Rye (Secale cereale) Seed Chitinase-c(Biochemistry & Molecular Biology)
• Localization, Accumulation, and Antifungal Activity of Chitinases in Rye (Secale cereale) Seed(Biochemistry & Molecular Biology)
• Isolation and Characterization of Chitinase Isoforms from the Bulbs of Four Species of the Genus Tulipa
• Complete Amino Acid Sequence of Chitinase-a from Bulbs of Gladiolus (Gladiolus gandavensis)
• Purification and Characterization of Two Chitinase Isoforms from the Bulbs of Gladiolus (Gladiolus gandavensis)
• The Amino Acid Sequence of Mitogenic Lectin-B from the Roots of Pokeweed : Phytolacca americana
• Superoxide Dismutase from the Silkworm, Bombyx mori : Sequence, Distribution, and Overexpression
• Utility of Dry Gel from Two-dimensional Electrophoresis for Peptide Mass Fingerprinting Analysis of Silkworm Proteins
• Integration of the twenty-fourth and twenty-seventh linkage groups of the silkwom, Bombyx mori
• Proteomic Studies of Isoforms of the P25 Component of Bombyx mori Fibroin
• 1P595 The protein rotational dynamics during the lysozyme prenucleation process(28. Macromolecular crystal growth,Poster Session,Abstract,Meeting Program of EABS & BSJ 2006)
• Thermal Unfolding Process of Dihydrolipoamide Dehydrogenase Studied by Fluorescence Spectroscopy
• Time-Resolved Fluorescence Studies on the Internal Motion of Chlorophyll a of Light-Harvesting Chlorophyll a/b-Protein Complex in Lipid Membranes
• Spectrally and Time-resolved Fluorescence Spectroscopic Study on Melittin-Calmodulin Interaction
• Kinetic Analysis of Lysozyme-Inhibitor Complex Formation
• On the Activation Process of Pepsinogen
• On the Mechanism of Lysozyme Catalysis II. Hydrolysis of Sugar Oxazoline
• Structural Properties of Silkworm Small Heat-Shock Proteins : sHSP19.9 and sHSP20.8
• Mitogenic Properties of Pokeweed Lectin-D Isoforms on Human Peripheral Blood Lymphocytes : Non-mitogen PL-D1 and Mitogen PL-D2
• Cloning, Sequencing, and Overexpression of Gene Encoding the Bacillus stearothermophilus Lipoate Acetyltransferase
• Application of the Fowler-Warten Method to a Stiff Enzymatic Reaction
• Effects of Carrageenans on the Binding, Phagocytotic, and Killing Abilities of Macrophages to Salmonella(Food & Nutrition Science)
• In vitro Bacteriostatic Effects of Dietary Polysaccharides
• 2P-091 蛍光法による酸性条件下での蛋白質変性中間体解析(蛋白質・物性(2),第46回日本生物物理学会年会)
• 2P-087 リフォールディングモモルチャリンの構造とダイナミクス(蛋白質・物性(2),第46回日本生物物理学会年会)
• The Unfolding of α-Momorcharin Proceeds Through the Compact Folded Intermediate
• Heterogeneous Packing in the Folding/Unfolding Intermediate State of Bitter Gourd Trypsin Inhibitor
• 2P010 Fluorescence spectroscopic study on the CaM-target protein interaction-Interaction with melittin(29. Protein structure and dynamics (II),Poster Session,Abstract,Meeting Program of EABS & BSJ 2006)
• Enzymatic System Realizing Two-factor Model
• Dynamic Characteristics of Enzymatic Feedback Systems
• Threshold of Enzymatic Reaction System, Further Studies with a Simplified Model
• Response of Enzymatic Feedback System to Oscillatory Input
• Oscillatory Behavior of Enzymatic Feedback System
• Studies on the Threshold Mechanism of Enzymatic Reaction
• Expression and Characterization of a Sigma-Class Glutathione S-Transferase of the Fall Webworm, Hyphantria cunea
• Partial Purification and Characterization of DOPA Quinone Imine Conversion Factor from Larval Hemolymph of the Silkworm, Bombyx mori
• Modification of Tryptophan Residues of Plant Class III Chitinases Involved in Enzyme Activity
• Positions of Disulfide Bonds in Rye (Secale cereale) Seed Chitinase-a
• Complete Amino Acid Sequence of Chitinase-A from Leaves of Pokeweed (Phytolacca americana)
• 1P047 Fluorescence spectroscopic characterization of folding/unfolding intermediate of ribosome-inactivation protein(Protein:Property,The 48th Annual Meeting of the Biophysical Society of Japan)
• The Quenching-Resolved Fluorescence Spectrum and Its Application to Studies of the Folding/Unfolding of Trypsin Inhibitor from Seeds of the Bitter Gourd
• Fluorescence Studies on the Lysozyme-Ligand Complex at 77K(Biological Chemistry)
• Thermally Induced Changes of Lipoate Acetyltransferase Inner Core Isolated from the Bacillus stearothermophilus Pyruvate Dehydrogenase Complex
• Computer Analysis of Lysozyme-catalyzed Reaction with Chitooligosaccharide
• Note on Steady-state Approximation of Enzyme Kinetics
• Simulation of Biochemical System with Computer Graphics
• Multiple conformational state of human serum albumin around single tryptophan residue at various pH revealed by time-resolved fluorescence spectroscopy
• Segmental Motion of Subsite C of Hen Egg-White Lysozyme : Fluorescence Depolarization Studies of Kyn62-Lysozyme as an Active Analogue
• The Steady State and Time-resolved Fluorescence Studies on the Lysozyme-Ligand Interaction
• Cloning, Sequencing, and Expression of the Tulip Bulb Chitinase-1 cDNA
• Complete Amino Acid Sequences of Chitinase-1 and -2 from Bulbs of Genus Tulipa
• A Ricin B chain-Ribonuclease A Hybrid Protein : A new tool for introduction of a foreign protein into targeted cells
• Base-catalyzed Intramolecular Rearrangement of Trimethylamine N-oxide
• 2H1512 Trp-Trp間エネルギー移動とPAPのfolding/unfolding研究への応用(蛋白質_物性3,第49回日本生物物理学会年会)
• Laser-Induced Chlorophyll Fluorescence Measurement System to Assess Photosynthetic Status within Leaves
• Laser-Induced Chlorophyll Fluorescence Measurement System to Assess Photosynthetic Status within Leaves
• 2E1610 Regulatory Mechanism of the fluorescence emission from Green Fluorescent Protein Chromophore(Proteins:Property II,Oral Presentation,The 50th Annual Meeting of the Biophysical Society of Japan)
• Fluorescence studies on the phase dependence of interactions between a tripeptide, lys-trp-lys, and dimyristoylphosphatidylserine.
• A Distinct Binding Mode of Archaeal Ribonuclease P Proteins to RNA

もっと見る 閉じる

スポンサーリンク