The Steady State and Time-resolved Fluorescence Studies on the Lysozyme-Ligand Interaction
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概要
- 論文の詳細を見る
The conformational change of hen egg-white lysozyme (EC 3.2.1.17) induced by the interaction with tri-N-acetyl-D-glucosamine were investigated by steady state and time-resolved fluorescence spectroscopy. To identify more clearly the conformation of hen egg-white lysozyme interacting with the ligand, the fluorescence decay kinetics of the lysozyme and its complex with the ligand were precisely measured at their full spectral regions. The spectral analysis based on the time-resolved studies showed that the binding of the ligand affected not only the Trp62 directly linked to the ligand but its influence was extended to the vicinity of Trp108 and further to the hydrophobic matrix box region. Near the binding site, the intramolecular distance between Trp108 and Glu35 was expanded or contracted depending on the pH of the buffer solution. On the other hand, the interaction of Trp28 and/or Trp111 with their surroundings was reduced by restriction of fluctuational motions at the hydrophobic matrix box region.
- 社団法人日本農芸化学会の論文
- 1995-07-23
著者
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Yamashita Shoji
Faculty Of Agriculture Graduate School Of Kyushu Univ
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Nishimoto E
Kurume Univ. School Of Medicine Fukuoka Jpn
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Yamasaki Nobuyuki
Laboratory Of Biochemistry Faculty Of Agriculture Graduate School Kyushu University
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Yamasaki Nobuyuki
Laboratory Of Biochemistry And Laboratory Of Protein Chemistry And Engineering Faculty Of Agricultur
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Yamasaki N
Laboratory Of Biochemistry Faculty Of Agriculture Graduate School Kyushu University
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Yamashita S
Institute Of Biophysics Faculty Of Agriculture Graduate School Of Kyushu University
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Nishimoto Etsuko
Institute Of Biophysics Faculty Of Agriculture Graduate School Of Kyushu University
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Yamashita Shoji
Institute Of Biophysics Faculty Of Agriculture Graduate School Of Kyushu University
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Yamasaki Nobuyuki
Laboratory Of Biochemistry Department Of Bioscience And Biotechnology Graduate School Faculty Of Agr
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Yamasaki Nobuyuki
Laboratory Of Agricultural Process Engineering Faculty Of Agriculture Kyushu University
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