Interactions of a Lysozyme-Monomethoxypolyethylene Glycol Conjugate with Lipopolysaccharides and Lipid Bilayers and Effects of Conjugate on Gram-negative Bacteria(Biochemistry & Molecular Biology)
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概要
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We have been studying a lysozyme derivative, called mPEG-lysozyme, in which Lys 33 is bound with a monomethoxypolyethylene glycol derivative. Here, we examined the surface hydrophobicity of the derivative and also its interactions with lipopolysaccharides and lipid bilayers. These properties may affect the antimicrobial activity of mPEG-lysozyme toward Gram-negative microorganisms. The lysozyme derivative had more than 150% of the antimicrobial activity for such microorganisms with that of native lysozyme taken to be 100%. Spectroscopic analyses indicated that mPEG-lysozyme bound to lipopolysaccharides with higher affinity than lysozyme, because of the high surface hydrophobicity of the derivative. In an experiment on carboxyfluorescein-leakage, mPEG-lysozyme strongly interacted with liposomes constructed from phosphatidylcholine, releasing carboxyfluorescein from the liposomes more effectively than lysozyme did. mPEG-lysozyme may perturb the outer membrane of Gram-negative microorganisms, gaining itself access to the peptidoglycan layers of the bacterium.
- 社団法人日本農芸化学会の論文
- 2002-09-23
著者
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Yamasaki Nobuyuki
Laboratory Of Biochemistry And Laboratory Of Protein Chemistry And Engineering Faculty Of Agricultur
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Iwasaki Kenta
Laboratory Of Biochemistry Division Of Bioresource And Bioenvironmental Sciences Graduate School Of
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Yamasaki Nobuyuki
Laboratory Of Biochemistry Division Of Bioresource And Bioenvironmental Sciences Graduate School Of
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Nodake Yuichi
Laboratory Of Biochemistry Department Of Pharmacy Faculty Of Pharmaceutical Sciences Nagasaki Intern
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Nodake Yuichi
Laboratory Of Biochemistry Division Of Bioresource And Bioenvironmental Sciences Graduate School Of
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Yamasaki Nobuyuki
Laboratory Of Agricultural Process Engineering Faculty Of Agriculture Kyushu University
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